Serum and follicular fluid concentration of stem cell factor in PCOS
DOI:
https://doi.org/10.18203/2320-1770.ijrcog20183754Keywords:
Clinical pregnancy rate, Polycystic ovary syndrome, Stem cell factorAbstract
Background: Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder in reproductive-aged women, which affects 5 to 20% of women in the reproductive age worldwide. This study aimed to compare the levels of SCF in serum and follicular fluid of PCOS patients with those of non-PCOS group and to investigate the relationship of SCF levels with ICSI success.
Methods: This is an observational case control study that included the patients who underwent ICSI in the Infertility-IVF center at Ankara University Faculty of Medicine and in a private IVF center between March 2016 and February 2017. The study group consisted of 57 PCOS patients diagnosed according to the Rotterdam criteria and the control group consisted of 75 patients with normofollicular and regular menstrual cycles. Serum and follicular fluid samples were taken on day of oocyte retrieval. Serum and follicular fluid SCF levels were determined by ELISA using the SCF ELISA kit..
Results: Serum and follicular fluid SCF levels in PCOS patients were found to be lower than in non-PCOS group. sSCF and ffSCF were statistically significantly higher who had clinical pregnancy than those who had no clinical pregnancy in the PCOS group.
Conclusions: SCF levels are low in serum and follicular fluid in patients with PCOS and that the increase in SCF levels is associated with an increase in oocyte maturation and clinical pregnancy rates in PCOS.
References
Trikudanathan S. Polycystic ovarian syndrome. Med Clin North America. 2014;99(1): 221-35.
Eshre TR, ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome. Fertil Steril. 2004;81(1), 19-25.
Eshre TT, ASRM-Sponsored PCOS Consensus Workshop Group. Consensus on infertility treatment related to polycystic ovary syndrome. Fertil Steril. 2008;89(3), 505-22.
Kalem, MN, Kalem Z, Gürgan T. Problems and Complications During the Treatment of Infertility in Women with Polycystic Ovary Syndrome. Gynecol Obstet Reprod Med, 2016;22(2):113-24.
De Leo V, Musacchio MC, Cappelli V, Massaro MG, Morgante G, Petraglia F. Genetic, hormonal and metabolic aspects of PCOS: an update. Reprod Biol Endocrinol. 2016;14(1):38.
Reddy P, Shen L, Ren C, Boman K, Lundin E, Ottander U, et al. Activation of Akt (PKB) and suppression of FKHRL1 in mouse and rat oocytes by stem cell factor during follicular activation and development. Develop Biol. 2005;281(2):160-70.
Liu K. Stem cell factor (SCF)-kit mediated phosphatidylinositol 3 (PI3) kinase signaling during mammalian oocyte growth and early follicular development. Front Biosci. 2006;11:126-35.
Raucci F, Di Fiore MM. Localization of c-kit and stem cell factor (SCF) in ovarian follicular epithelium of a lizard, Podarcis s. sicula. Acta Histochemica. 2011;113(6):647-55.
Zhang X, Zhang H, Gao Q, Ji S, Bing L, Hao J. Sohlh2 inhibits the apoptosis of mouse primordial follicle oocytes via C-kit/PI3K/Akt/Foxo3a signalling pathway. Reprod Biomed Online. 2015;30(5):514-21.
Hu R, Wang FM, Yu L, Luo Y, Wu X, Li J, Zhang XM, Oehninger S, Bocca S. Antimüllerian hormone regulates stem cell factor expression in human granulosa cells. Fertil Steril. 2014;102(6):1742-50.
Jin X, Han CS, Yu FQ, Wei P, Hu ZY, Liu YX. Anti‐apoptotic action of stem cell factor on oocytes in primordial follicles and its signal transduction. Mol Reprod Develop: Incorporating Gamete Res. 2005;70(1):82-90.
Tan J, Wen XY, Su Q, Huang ZH, He JX, Xin CL, Wu QF, Zou Y. Reduced expression of SCF in serum and follicle from patients with polycystic ovary syndrome. Eur Rev Med Pharmacol Sci. 2016;20(24):5049-57.
Tan J, Zou Y, Wu XW, Tian LF, Su Q, et al. Increased SCF in follicular fluid and granulosa cells positively correlates with oocyte maturation, fertilization, and embryo quality in humans. Reprod Sci. 2017;24(11):1544-50.
Qiao J, Feng HL. Extra-and intra-ovarian factors in polycystic ovary syndrome: impact on oocyte maturation and embryo developmental competence. Hum Reprod Update. 2010;17(1):17-33.
Ozeren M, Dinc H, Ekmen U, Senekayli C, Aydemir V. Umbilical and middle cerebral artery Doppler indices in patients with preeclampsia. Eur J Obstet Gynecol Reprod Biol. 1999;82:11-6.
Child TJ, Abdul-Jalil AK, Gulekli B, Tan SL. In vitro maturation and fertilization of oocytes from unstimulated normal ovaries, polycystic ovaries, and women with polycystic ovary syndrome. Fertil Steril. 2001;76(5):936-42.
Mikkelsen AL, Lindenberg S. Benefit of FSH priming of women with PCOS to the in vitro maturation procedure and the outcome: a randomized prospective study. Reproduction. 2001;122(4):587-92.
Sun LJ, Sun HX, Qian QR, Yue LF, Wang XL, Guan YC, et al. Correlationship between SCF, TGF-βl level in serum and follicular fluid with PCOS pathogenesis and IVF outcome In PCOS patients [J]. Chinese J Birth Health Heredity. 2012;10:053.
Smikle CB, Dandekar PV, Schriock ED, Givens CR. Elevated ovarian follicular fluid stem cell factor concentrations are associated with improved pregnancy rates in in-vitro fertilization cycles. Fertil Steril. 1998;69(1):70-2.
Gizzo S, Quaranta M, Andrisani A, Bordin L, Vitagliano A, Esposito F, et al. Serum stem cell factor assay in elderly poor responder patients undergoing IVF: a new biomarker to customize follicle aspiration cycle by cycle. Reprod Sci. 2016;23(1):61-8.
