Oocyte central granularity and its relation to pregnancy outcome following intracytoplasmic sperm injection-freeze-all cycles

Aakriti Gupta; Alex C. Vargese; Mohamed C. Ashraf; Dhannya Binoy

doi:10.18203/2320-1770.ijrcog20222303

Authors

Aakriti Gupta Department of Obstetrics and Gynecology, SGRR Medical College and Hospital, Dehradun, Uttarakhand, India
Alex C. Vargese Craft Hospital and Research Centre, Thrissur, Kerala, India
Mohamed C. Ashraf Department of Reproductive Medicine, Craft Hospital and Research Centre Thrissur, Kerala, India
Dhannya Binoy Craft Hospital and Research Centre, Thrissur, Kerala, India

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20222303

Keywords:

ICSI, FET, Oocyte dense central granularity, Pregnancy outcomes

Abstract

Background: Oocyte quality is instrumental in the development of early embryos and may account for the difference in the rates of fertilization, cleavage, implantation and pregnancy outcome. Centrally located cytoplasmic granularity is a rare morphological feature that can be observed in certain cases. We conducted this study with the sole aim to compare the pregnancy outcomes from embryo derived exclusively from oocytes with dense central granularity with embryos derived from oocytes with normal morphology in intracytoplasmic sperm injection (ICSI)-freeze- all cycles.

Methods: A retrospective case control study performed during a period of 1 year and 10 months from Jan 2018-Oct 2019 at craft hospital and research centre India; a tertiary heath care centre. The 62 infertile women undergoing ICSI FET cycle with oocyte cohort having all central granularity oocytes were included as study participants after fulfilling the inclusion and exclusion criteria. The 200 women undergoing ICSI- frozen embryo transfer (FET with normal morphology oocyte were taken. Implantation rate and clinical pregnancy rate were evaluated as primary outcomes. Ongoing pregnancy rate, live birth rate, fertilization rate and top-quality embryo were taken as secondary outcomes.

Results: The implantation rate and clinical pregnancy rate was 21.48% and 40.33% in study group compared to 35.012% and 58.5% respectively in control group which was statistically significant (p=0.002; 0.012 respectively).

Conclusions: Presence of dense central granularity in the oocyte can be used to predict poor ART outcome in terms of low implantation and clinical pregnancy rates.

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Author Biographies

Aakriti Gupta, Department of Obstetrics and Gynecology, SGRR Medical College and Hospital, Dehradun, Uttarakhand, India

obstetrics and gynaecology

assistant professor

Alex C. Vargese, Craft Hospital and Research Centre, Thrissur, Kerala, India

Head Embryology

Craft Hospitala nd reseach institute kerala

References

Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul Consensus Workshop on Embryo Assessment: Proceedings of an Expert Meeting. Hum Reprod. 2011;26:1270-83.

De Shutter P, Dozortsev D, Qian C, Dhont M. Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmatic sperm injection. Hum Reprod. 1996;11:595-7.

Veeck LL. Oocyte quality and assisted conception. Acta Eur Fertil. 1992;23:275-88.

Rienzi L, Vajta G, Ubaldi F. Predictive value of oocyte morphology in human IVF: a systematic review of the literature. Hum Reprod Update. 2010;12.

De Sutter P, Dozortsev D, Qian C, Dhont M. Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection. Hum Reprod. 1996;11:595-7.

Ebner T, Moser M, Tews G. Is oocyte morphology prognostic of embryo developmental potential after ICSI. RBM. 2006;12(4):507-12.

Serhal PF. Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Human Reprod. 1997;12:1267-70.

Fancsovits P, Tothne ZG, Murber AJr, Urbancsek J. Importance of cytoplasmic granularity of human oocytes in vitro fertilization. Acta Biol Hung. 2012;63(2):189-201.

Sun Y, Wang F. The effects of the down-regulation on quality of oocyte. J Reprod Med (Chinese). 2007;16:327-8 .

Kahraman S. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Human Reprod. 2000;15:2-2393.

Merviel P. Impact of oocytes with CLCG on ICSI outcomes and their potential relation to pesticide exposure. J Ovarian Res. 2017;10(1):42.

Esfandiari N, Burjaq H, Gotlieb L, Casper RF. Brown oocytes: implications for assisted reproductive technology. Ferti steril. 2006;86(5):1522-5.

Van Blerkom J, Henry G. OOcyte dysmorphism and aneuploidy in meiotically mature human oocytes after ovarian stimulation. Hum Reprod. 1992;7:379-90.

Halliday J. Outcomes of IVF conceptions: are they different? Best Pract Res Clin Obstet Gynaecol. 1997;21:67-81.

Niemitz EL, Feinberg AP. Epigenetics and assisted reproductive technology: a call for investigation. Am J Hum Genet. 2004;74(4):599-609.

Xiao-Fang Y, Hong-Lin X. Relationship between the positions of cytoplasmic granulation and oocytes developmental potential in human. Sci Rep. 2019;9(1):7215.