Profile of patients with HPV infection at the regional hospital of Saint-Louis, Senegal
DOI:
https://doi.org/10.18203/2320-1770.ijrcog20232924Keywords:
HPV typing, Saint Louis regional hospital, Cervical cancer screeningAbstract
Background: Objectives of current study was to define the sociodemographic characteristics of patients, to determine the frequency of HPV infection at the CHRSL and to describe factors associated with HPV infection.
Methods: This was a prospective cross-sectional study conducted in the gynecology-obstetrics department of the Saint Louis Regional Hospital Center from November 11, 2019 to November 11, 2021, a period of 24 months. We studied sociodemographic, clinical and test results characteristics. Samples were taken from patients in the gynaecological position using a dedicated kit (cytobrush and tube). Viral research was carried out using the Atila Biosystems device for amplification and detection of viral DNA. This test genotypes HPV 16, 18 and 45, and detects 12 other HR HPVs (31, 33, 35, 39, 51, 52, 56, 58, 59, 66 and 68) simultaneously using three probes labelled with different fluorophores. Analysis was performed using Epi-Info software and Excel 2010.
Results: The study population comprised 128 patients. The mean age of the patients was 41.95 years, with extremes of 23 and 70 years. They were married (92.97%) and housewives (46.88%). Almost all patients (77.34%) were genitally active. The average age at marriage was 21.78 years, and polygamous households predominated (53.91%). The average age at first intercourse was 20.05 years. The average age at first pregnancy was 21.78. Over (57.81%) of patients had at least two partners. Incense was used in 97.66% of cases. The viral HPV test was positive in (38.28%) of patients. High-risk papillomaviruses were the most common, at 63.27%. Colposcopy was performed in 17.19%; normal and satisfactory in (63.64%) of patients, with 22.27% of cervical biopsies, and histology showed one CIN2 and two CIN3. Therapeutically, one thermoablation and two hysterectomies were performed.
Conclusions: HPV viral typing in primary screening for cervical cancer offers opportunities and remains realistic and feasible in less developed countries such as Senegal, despite modest resources.
References
Bruni L, Barrionuevo-Rosas L, Albero G, Aldea M, Serrano B, Valencia S, et al. ICO Information Centre on HPV and Cancer (HPV Information Centre). Human Papillomavirus Relate Dis. 2015;3:20.
Zohoncon TM, Ouedraogo TC, Brun LVC, et al. Molecular Epidemiology of High-Risk Human Papillomavirus in High-Grade Cervical Intraepithelial Neoplasia and in Cervical Cancer in Parakou, Republic of Benin. Pak J Biol Sci. 2016;19:49-56.
Mor C. Le test viral HPV en dépistage primaire du cancer du col de l’uterus : à propos de 144 cas au centre de sante Nabil Choucair et à l’hôpital militaire de Ouakam (Dakar, Sénégal). Thèse Med. 2019.
Duport N. Institut de Veille Sanitaire. Données épidémiologiques sur le cancer du col de l’utérus. Etat des Connai. 2008;3:33.
Information centre on HPV and cancer Sénégal: Human Papillomavirus and related cancers fact sheet. Available at: https://www.who.int. Accessed on 20 September 2022.
N’Golet A, Koutoupot BR, Lubuélé L. Cervical intraepithelial neoplasia (CIN) in Brazzaville, Congo. Ann Pathol. 2004;24:324-8.
Traore IMA, Zohoncon TM, Dembele A. Molecular characterization of high-risk human papillomavirus in women in Bobo-Dioulasso, Burkina Faso. BioMed Res Int. 2016.
Cattani P, Siddu A, D’Onghia S. RNA (E6 and E7) Assays versus DNA (E6 and E7) Assays for Risk Evaluation for Women Infected with Human Papillomavirus. J Clin Microbiol. 2009;47:2136-41.
Traore S. Le dépistage des CIN par l’inspection visuelle à l’acide acétique et du Lugol : à propos de 4632 femmes dépistées dans le district de Bamako. Faculté Méd Pharma Odontostomatol. Thèse Année. 2004;11:53-9.
Biaye B. Dépistage du cancer du col de l’utérus par méthodes visuelles au senegal: experience du centre de sante Nabil Choucair de Dakar, Sénégal. Thèse Méd Univ Cheikh Anta J. 2018:38:45-8.
Sophie I. Place de la vaccination antipapillomavirus humains dans la prevention du cancer du col de l’utérus : situation a l’ile de la reunion. These Pharm Nancy Univ Lorraine J. 2012.
Samaké B. Lesions précancéreuses et cancéreuses du col de l’utérus au centre de santé de reférence de la commune V du district de Bamako. Thèse Méd Fmpos Bamako Année. 2010;47:102-7.
Mergui JL. Quel suivi après traitement chirurgical d’une lésion de haut grade du col utérin ? Gynécol Obstét Fertil. 2008;36:441.
Sankaranarayanan R, Nene BM, Shastri SS. HPV Screening for Cervical Cancer in Rural India. N Engl J Med. 2009;360:1385-94.
Holmes RS, Hawes SE, Touré P. HIV infection as a risk factor for cervical cancer and cervical intraepithelial neoplasia in Senegal. Cancer Epidemiol Biomark Prev Publ Am Assoc Cancer Res. 2009;18: 2442-6.
Lo S, Thiam O, Sow DB. Diagnostic et identification des HPV à haut risque par PCR multiplex au Centre Hospitalier Régional de Saint-Louis du Sénégal. J Clin Microbiol. 2020;23:12-8.
Direction Generale de la Sante, Comite technique des vaccinations. Guide des vaccinations. Saint-Denis. Available at: https://sante.gouv.fr/IMG/pdf/Guide_ des_vaccinations_edition_2012.pdf. Accessed on 20 February 2023.
Tawil S. Les freins à la vaccination contre les papillomavirus : enquête chez les parents de jeunes filles de 11 à 19 ans. Thèse Med Paris Univ J. 2015;7: 24.
Gassama O. Bilan des activités de colposcopie au service de gynécologie-obstétrique du CHU Aristide Le Dantec. Fmpos Dakar Année. 2011;43:22-6.
Schockaert S, Poppe W, Arbyn M. Incidence of vaginal intraepithelial neoplasia after hysterectomy for cervical intraepithelial neoplasia: a retrospective study. Am J Obstet Gynecol. 2008;199:1-5.
Bardin A, Vaccarella S, Clifford GM. Human papillomavirus infection in women with and without cervical cancer in Warsaw, Poland. Eur J Cancer Oxf Engl. 2008;44:557-64.
Haute A. Recommandations en santé publique : état des lieux et recommandations pour le dépistage du cancer du col de l’utérus en France. HAS. 2010;1:256.
Hantz S, Alain S, Denis F. Vaccins prophylactiques anti-papillomavirus : enjeux et perspectives. Gynecol Obstet Fertil. 2006;34(7-8):647-55.
