Blastulation rate using fresh versus devitrified oocytes using homologous and heterologous sperm in an assisted reproduction clinic in a middle-income country

Jaime Antonio Hidalgo-Carrera; Jorge L. Lezama-Ruvalcaba; Carlos G. Salazar-Lopez Ortiz; Jose Carlos Salazar-Trujillo; Ricardo Rodriguez-Calderon; Sergio Tellez-Velasco; Fernando Mancilla-Hernandez

doi:10.18203/2320-1770.ijrcog20241419

Authors

Jaime Antonio Hidalgo-Carrera Department of Gynecology, Hospital Español Fertility Clinic, HISPAREP, Mexico City, Mexico https://orcid.org/0000-0002-5384-3015
Jorge L. Lezama-Ruvalcaba Department of Gynecology, Hospital Español Fertility Clinic, HISPAREP, Mexico City, Mexico
Carlos G. Salazar-Lopez Ortiz Department of Gynecology, Hospital Español Fertility Clinic, HISPAREP, Mexico City, Mexico
Jose Carlos Salazar-Trujillo Department of Gynecology, Hospital Español Fertility Clinic, HISPAREP, Mexico City, Mexico
Ricardo Rodriguez-Calderon Department of Gynecology, Hospital Español Fertility Clinic, HISPAREP, Mexico City, Mexico
Sergio Tellez-Velasco Department of Gynecology, Hospital Español Fertility Clinic, HISPAREP, Mexico City, Mexico
Fernando Mancilla-Hernandez Department of Gynecology, Hospital Español Fertility Clinic, HISPAREP, Mexico City, Mexico

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20241419

Keywords:

ICSI, Fresh oocytes, Vitrified oocytes, Blastocyst, Homologous semen, Heterologous semen

Abstract

Background: Assisted reproduction has revolutionized the field of reproductive medicine, giving hope to many couples who face difficulties conceiving naturally. In this case, fresh oocytes and devitrified oocytes play a fundamental role in fertility treatments.

Methods: Descriptive, retrospective and observational study in which the records of the HISPAREP reproduction clinic of the Spanish hospital were analyzed in the period from 2020 to 2022.

Results: A total of 136 blastocysts that reached day 5 were studied compared to 8 embryos that were blocked before reaching blastulation. Of this, 76 oocytes were fresh, of which 97.4% reached day 5 (74) and the remaining two were blocked. Of the vitrified ones, a total of 68 were devitrified and of these 91.2% reached D5. Of the correctly fertilized oocytes, 96.5% (136) reached blastocysts compared to 5 that were blocked and 3 that were blocked from those that were not fertilized. Regarding the fertilization rate of fresh oocytes, a total of 76 was obtained, which is equivalent to 100%, and of the devitrified oocytes, 65 were fertilized (95.6%) and 3 were not fertilized (4.4%). With a variation of 6.2% in the blastocyst rate, between fresh and devitrified oocytes.

Conclusions: There is no significant difference in the reproductive results of fresh and vitrified oocytes. It is necessary to try to carry out more studies with a larger sample size to obtain more conclusive results. The reproductive outcomes of fresh oocytes and oocytes devitrified using fresh sperm and frozen sperm are similar. With this, fresh oocytes have a slightly higher probability of implantation.

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