Evaluating the efficacy of Dfrag tablets on human sperm DNA fragmentation - insights from a prospective clinical study

Authors

  • Mala Raj Infertility Specialist, Endoscopic Surgeon, Firm Hospitals, Chennai, Tamil Nadu, India
  • Roobini J. Clinical Embryologist, Firm Hospitals, Chennai, Tamil Nadu, India
  • Aarathy Kasturiraj Clinical Embryologist, Firm Hospitals, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20241772

Keywords:

Early clinical exposure, Knowledge, Medical education, Medical students

Abstract

Background: Infertility affects approximately 8-10% of couples worldwide. Sperm deoxyribonucleic acid (DNA) fragmentation index (DFI) has emerged as a significant factor in infertility research, highlighting its importance in understanding reproductive health.

Methods: This prospective clinical study aimed to assess the impact of Dfrag® tablets, a unique nutraceutical combination containing vitamin D3 (600 IU), selenomethionine (40 mcg), coenzyme Q10 (100 mg), and astaxanthin (8 mg), on high sperm DFI over a 3-month period. The study utilized the sperm chromatin dispersion test (SCD) to measure DFI and examined semen parameters before and after the intervention.

Results: Dfrag® tablets were found to significantly improve semen volume, total sperm count, sperm concentration, and progressive motility within the 3-month treatment period. The study reported an average reduction of 36% in DNA fragmentation levels post-treatment with Dfrag® tablets. However, no significant changes were observed in total motility or sperm morphology.

Conclusions: This study demonstrates the potential of Dfrag® tablets in reducing sperm DNA fragmentation and improving key semen parameters associated with fertility.

 

References

World Health Organization. Programme of Maternal and Child Health and Family Planning Unit. Infertility: a tabulation of available data on prevalence of primary and secondary infertility. 1991. Available ta: https://iris.who.int/handle/10665/59769. Accessed on 08 May 2024.

Poongothai J, Gopenath TS, Manonayaki S. Genetics of human male infertility. Singapore Med J. 2009;50:336-47.

Chander PP, Indira H, Kusum Z. Need and feasibility of providing assisted technologies for infertility management in resource poor settings. ICMR Bull. 2000;30:55-62.

Mascarenhas MN, Flaxman SR, Boerma T, Vanderpoel S, Stevens GA. National, regional, and global trends in infertility prevalence since 1990: A systematic analysis of 277 health surveys. PLoS Med. 2012;9:e1001356.

Thonneau P, Marchand S, Tallec A, Ferial ML, Ducot B, Lansac J, et al. Incidence and main causes of infertility in a resident population (1,850,000) of three French regions (1988-1989). Hum Reprod. 1991;6:811-6.

Evenson DP, Jost LK, Marshall D, Zimaman MJ, Clegg E, Purvis K, de Angelis P, Claussen OP. Utility of sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Human Reproduction. 1999; 14: 1039–1049.

Larson KL, Brannian JD, Singh NP, Burbach JA, Jost LK, Hansen KP, et al. Chromatin structure in globozoospermia: a case report. J Androl. 2001;2:424-31.

Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi PG, Bianchi U: Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod. 1999;4:31-7.

Morris ID, Ilott S, Dixon L, Brison DR. The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development. Hum Reprod. 2002;17:990-8.

Zini A, Meriano J, Kader K, Jarvi K, Laskin CA, Cadesky K. Potential adverse effect of sperm DNA damage on embryo quality after ICSI. Hum Reprod. 2005;20:3476-80.

Denschlag D, Tempfer C, Kunze M, Wolff G, Keck C. Assisted reproductive techniques in patients with Klinefelter syndrome: a critical review. Fertil Steril. 2004;82:775-9.

Visser L, Westerveld GH, Korver CM, van Daalen SK, Hovingh SE, Rozen S, et al. Y chromosome gr/gr deletions are a risk factor for low semen quality. Hum Reprod. 2009;24:2667-73.

Pourmasumi S, Sabeti P, Rahiminia T, Mangoli E, Tabibnejad N, Talebi AR. The etiologies of DNA abnormalities in male infertility: An assessment and review. Int J Reprod Biomed. 2017;15(6):331-44.

Alahmar AT. Role of Oxidative Stress in Male Infertility: An Updated Review. J Hum Reprod Sci. 2019;12(1):4-18.

Ko EY, Sabanegh ES, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril. 2014;102:1518-27.

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Published

2024-06-27

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Original Research Articles