Analysing the DNA integrity in hypo-osmotic swelling test types of tail curling and assessing if HOS type “B” outweighs the rest

Swathy Yuvaraj; Manjula G.; Radha Vembu; Sanjeeva Reddy; Monna Pandurangi

doi:10.18203/2320-1770.ijrcog20243189

Authors

Swathy Yuvaraj Department of Reproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India
Manjula G. Department of Reproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India
Radha Vembu Department of Reproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India
Sanjeeva Reddy Department of Reproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India
Monna Pandurangi Department of Reproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20243189

Keywords:

Asthenozoospermia, DNA fragmentation, HOS test, HOS type B tail curling

Abstract

Background: One of the important steps in ICSI is to select a sperm with good DNA content to have a successful out-come. In case of complete asthenozoospermia the aim is to select a viable sperm using the hypo-osmotic swelling test. There has been lot of debate regarding type of tail curling and their DNA integrity. This study aimed to analyze DNA integrity among different hypo-osmotic swelling (HOS) types of tail curling and determine whether HOS type B has superior DNA quality compared to other types.

Methods: A total of 43 semen samples were collected from patients who attend a tertiary care centre in Chennai. The samples were subjected to HOS testing, followed by DNA integrity assessment using acridine orange staining.

Results: The study found that sperm with HOS type B tail curling exhibited significantly lower DNA fragmentation (24.6%) compared to HOS type A (97.7%) and other types (61.9%), suggesting that HOS type B sperm possess better DNA integrity.

Conclusions: These findings propose that selecting HOS type B sperm in cases of asthenozoospermia for intracytoplasmic sperm injection (ICSI) could enhance outcomes, as this method is cost-effective and identifies sperm with good DNA content.

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