A comparative study of various diagnostic modalities for female genital tuberculosis in infertility in a tertiary hospital
DOI:
https://doi.org/10.18203/2320-1770.ijrcog20243156Keywords:
GTB, Infertility, Diagnostic modalitiesAbstract
Background: Infertility is a complex and emotionally challenging issue that affects millions of people worldwide. Infertility has a detrimental effect on women's social standing and psychological health. The aim of this study is to compare the effectiveness of several diagnostic techniques for female genital tuberculosis (TB) in infertility. Objectives to study various diagnostic techniques in patients with infertility attending Obstetrics and Gynaecology outpatient department (OPD) of Rohilkhand Medical College and Hospital (RMCH), Bareilly.
Methods: This was a one-year hospital based cross-sectional comparative study carried out in Department of Obstetrics and Gynaecology, RMCH, Bareilly, from 01 November 2019 to 31 October 2020 in women with age group 18-45 years presented with indications of primary and secondary infertility and the estimated sample size was 50.
Results: Highest percentage of cases (48%) have been reported for 21-30 age group, least below 20 age group (2%). The detection rates for acid-fast bacilli (AFB) smear, Lowenstein-Jensen (L-J) culture, Mycobacteria growth indicator tube (MGIT) culture and histopathological examination (HPE), were 4%, 4%, 16% and 12%, respectively. Accuracy of MGIT-960 culture correlating with HPE was found to be 92%. The overall comparative analysis between different methods of diagnosis showed that sensitivity was highest for MGIT-960 (62.5%), however, specificity was found to be 100% for both AFB and LJ culture, while 97.6% for MGIT-960 culture.
Conclusions: Our results indicate that MGIT-960 is an efficacious detection strategy for GTB, in cases of female infertility.
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References
Aliyu MH, Aliyu SH, Salihu HIM. Female genital tuberculosis: a global review. Int J Fertil Womens Med. 2004;49(3):123-36.
Bose M. Female genital tract tuberculosis: how long will it elude diagnosis? Indian J Med Res. 2011;134:13-4.
Xiang W, Liao, ACR and Chan, C. A new molecular variant of Luteinizing Hormone associated with female infertility. Fertil Steril. 1998;69:102.
Sharma JB. Current Diagnosis and Management of Female Genital Tuberculosis. J Obstet Gynaecol India. 2015;65(6):362-71.
Sharma D, Depan A, Yadav K, Narayan S, Sharma A. Prevalence of female genital tract tuberculosis in suspected cases attending Gynecology OPD at tertiary centre by various diagnostic methods and comparative analysis. Int J Reprod Contracept Obstet Gynecol. 2019;8:2286-92.
Jindal N, Gainder S, Dhaliwal LK, Sethi S. The Role of MGIT 960 Culture Medium in Resolving the Diagnostic Dilemma for Genital Tuberculosis Patients Presenting with Infertility. J Obstet Gynaecol India. 2018;68(2):123.
Ali AA, Abdallah TM. Clinical presentation and epidemiology of female genital tuberculosis in eastern Sudan. Int J Gynaecol Obstet. 2012;118(3):236-8.
Goel G, Khatuja R, Radhakrishnan G, Agarwal R, Agarwal S, Kaur I. Role of newer methods of diagnosing genital tuberculosis in infertile women. Indian J Pathol Microbiol. 2013;56:155.
Potter J, Leddy S, Kunst H, White V. P184 Female Genital Tuberculosis: The Long Road To Diagnosis. Thorax. 2014;69:A156-7.
Mittal P, Gupta S. A comparative study for detection of mycobacteria by direct AFB smear examination, culture by Lowenstein Jensen media, fluorescent sensor technology based BACTEC, micro MGIT system & PCR. Int J Med Sci Educ. 2016;3(3):257-63.
Shrivastava G, Bajpai T, Bhatambare GS, Patel KB. Genital tuberculosis: Comparative study of the diagnostic modalities. J Hum Reprod Sci. 2014;7(1):30-3.
Alan JW, Louis RK, Andrew CN, Partin AW, Peters CA. Campbell-Walsh Urology. 9th Edition. New York: Saunders, Elsevier. 2006.
Sorlozano A, Soria I, Roman J, Huertas P, Soto MJ, Piedrola G, et al. Comparative evaluation of three culture methods for the isolation of mycobacteria from clinical samples. J Microbiol Biotechnol. 2009;19(10):1259-64.
Rodrigues C, Shenai S, Sadani M, Sukhadia N, Jani M, Ajbani K, et al. Evaluation of the bactec MGIT 960 TB system for recovery and identification of Mycobacterium tuberculosis complex in a high through put tertiary care centre. Indian J Med Microbiol. 2009;27(3):217-21.
Chaman-Ara K, Bahrami MA, Bahrami E, Bahrami S, Bahrami MN, Moosazadeh M, et al. Prevalence of genital tuberculosis among infertile women: a systematic review and meta-analysis. Int J Med Res Health Sci. 2016;5(4):208-15.