Evaluation of the synergistic application of bone marrow aspirate concentrate and platelet-rich plasma therapy in women experiencing premature ovarian failure and poor ovarian reserve: a case series

Authors

  • Vyjayanthi Srinivasan KIMS Fertility Centre, Krishna Institute of Medical Sciences, Secunderabad, Telangana, India
  • Sharada Golconda KIMS Fertility Centre, Krishna Institute of Medical Sciences, Secunderabad, Telangana, India
  • Kurapati Krishnaiah Department of Orthopedics, Krishna Institute of Medical Sciences, Secunderabad, Telangana, India

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20253101

Keywords:

Bone marrow derived aspirate concentrate, Platelet rich plasma, BMAC, PRP, Poor ovarian response, Premature, Ovarian failure, In vitro fertilization

Abstract

Autologous bone marrow-derived stem cells (BMSCs) and platelet-rich plasma (PRP) separately were found to be effective in restoring ovarian function in infertile women. The present study aims to study the synergistic effect of bone marrow-derived aspirate concentrate (BMAC) and PRP in women diagnosed with poor ovarian response (POR) and premature ovarian failure (POF) undergoing in vitro fertilization (IVF). A total of 10 patients participated in the study. Eight patients were diagnosed with POR, while two patients had POF. Intraovarian BMAC-PRP injection was given to all the patients via the laparoscopic or transvaginal route. Group 1 before starting the IVF cycle, group 2-intracycle-post ovum pick-up and group 3-POF patients. Outcomes assessed include improvement in antral follicular count (AFC), Anti-Mullerian hormone (AMH), response to ovarian stimulation at subsequent IVF cycle and clinical pregnancy.  In group I POR patients (n=4) post-instillation, an increase in AFC count was observed and subsequently, these patients underwent IVF, however, no clinical pregnancies were achieved. Group II POR patients (n=4), who received BMAC-PRP instillation intracycle post-OPU there was improvement in AFC and better response with more oocytes and embryos in the subsequent IVF cycle, leading to three pregnancies in this group. In group III-POF patients (n=2) who received BMAC-PRP instillation, one of them spontaneously conceived post 3 months of instillation. The synergistic application of BMAC-PRP was found to be effective in improving ovarian function in POR and POF patients.

 

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References

Kou M, Huang L, Yang J, Chiang Z, Chen S, Liu J, et al. Mesenchymal stem cell-derived extracellular vesicles for immunomodulation and regeneration: a next generation therapeutic tool? Cell Death Dis. 2022;13(7):580. DOI: https://doi.org/10.1038/s41419-022-05034-x

Jin Y, Li S, Yu Q, Chen T, Liu D. Application of stem cells in regeneration medicine. MedComm. 2023;4(4):e291. DOI: https://doi.org/10.1002/mco2.291

Cucinella G, Gullo G, Catania E, Perino A, Billone V, Marinelli S, et al. Stem Cells and Infertility: A Review of Clinical Applications and Legal Frameworks. JPM. 2024;14(2):135. DOI: https://doi.org/10.3390/jpm14020135

Liu J, Zhang H, Zhang Y, Li N, Wen Y, Cao F, et al. Homing and Restorative Effects of Bone Marrow-Derived Mesenchymal Stem Cells on Cisplatin Injured Ovaries in Rats. Molecules and Cells. 2014;37(12):865-72. DOI: https://doi.org/10.14348/molcells.2014.0145

Jing Z, Qiong Z, Yonggang W, Yanping L. Rat bone marrow mesenchymal stem cells improve regeneration of thin endometrium in rat. Fertility and Sterility. 2014;101(2):587-94. DOI: https://doi.org/10.1016/j.fertnstert.2013.10.053

Everts P, Onishi K, Jayaram P, Lana JF, Mautner K. Platelet-Rich Plasma: New Performance Understandings and Therapeutic Considerations in 2020. IJMS. 2020;21(20):7794. DOI: https://doi.org/10.3390/ijms21207794

Moustakli E, Potiris A, Zikopoulos A, Zachariou A, Topis S, Panagopoulos P, et al. Platelet-Rich Plasma (PRP) in Reproductive Medicine: A Critical Review of PRP Therapy in Low-Reserve and Premature Ovarian Insufficiency. Biomedicines. 2025;13(5):1257. DOI: https://doi.org/10.3390/biomedicines13051257

Anagani M, Agrawal P, Reddy BV, Mishra PC. Role of autologous bone marrow derived stem cells and platelet rich plasma for endometrial regeneration and repair and ovarian rejuvenation. Int J Reprod Contracept Obstet Gynecol. 2021;10(2):597. DOI: https://doi.org/10.18203/2320-1770.ijrcog20210311

Qian Y, Han Q, Chen W, Song J, Zhao X, Ouyang Y, et al. Platelet-Rich Plasma Derived Growth Factors Contribute to Stem Cell Differentiation in Musculoskeletal Regeneration. Front Chem. 2017;5:89. DOI: https://doi.org/10.3389/fchem.2017.00089

Tandulwadkar S, Mishra S, Jain H, Mane N. Ray of Hope for Decreased Ovarian Reserve: India’s First Two Spontaneous Conceptions After Autologous Bone Marrow-Derived Stem Cells Therapy for Ovarian Rejuvenation. J Obstet Gynecol India. 2022;72(S2):458-60. DOI: https://doi.org/10.1007/s13224-022-01682-3

Tandulwadkar S, Karthick Ms. Combined use of autologous bone marrow-derived stem cells and platelet-rich plasma for ovarian rejuvenation in poor responders. J Hum Reprod Sci. 2020;13(3):184. DOI: https://doi.org/10.4103/jhrs.JHRS_130_19

Ghodake V. Clinical Approach of Novel Autologous Stem Cell Therapy for Primary Ovarian Insufficiency and Endometrial Regeneration. SRDT. 2022;8(3):1-6. DOI: https://doi.org/10.24966/SRDT-2060/100102

Cakiroglu Y, Saltik A, Yuceturk A, Karaosmanoglu O, Kopuk SY, Scott RT, et al. Effects of intraovarian injection of autologous platelet rich plasma on ovarian reserve and IVF outcome parameters in women with primary ovarian insufficiency. Aging. 2020;12(11):10211-22. DOI: https://doi.org/10.18632/aging.103403

Pantos K, Simopoulou M, Pantou A, Rapani A, Tsioulou P, Nitsos N, et al. A Case Series on Natural Conceptions Resulting in Ongoing Pregnancies in Menopausal and Prematurely Menopausal Women Following Platelet-Rich Plasma Treatment. Cell Transplant. 2019;28(9-10):1333-40. DOI: https://doi.org/10.1177/0963689719859539

Herraiz S, Buigues A, Díaz-García C, Romeu M, Martínez S, Gómez-Seguí I, et al. Fertility rescue and ovarian follicle growth promotion by bone marrow stem cell infusion. Fertility and Sterility. 2018;109(5):908-18. DOI: https://doi.org/10.1016/j.fertnstert.2018.01.004

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Published

2025-09-26

How to Cite

Srinivasan, V., Golconda, S., & Krishnaiah, K. (2025). Evaluation of the synergistic application of bone marrow aspirate concentrate and platelet-rich plasma therapy in women experiencing premature ovarian failure and poor ovarian reserve: a case series. International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 14(10), 3516–3520. https://doi.org/10.18203/2320-1770.ijrcog20253101

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Case Series