Assessment of the relationship between follicular fluid calcium ion concentration and reproductive outcomes

Authors

  • Özkan Özdamar Department of Obstetrics and Gynecology, Gölcük Military Hospital, Kocaeli, Turkey
  • İsmet Gün Department of Obstetrics and Gynecology, Zeynep Kamil Maternity and Children’s Hospital, Istanbul, Turkey
  • Serdar Hira Department of Obstetrics and Gynecology, Zeynep Kamil Maternity and Children’s Hospital, Istanbul, Turkey
  • Belgin Devranoğlu Department of Obstetrics and Gynecology, Zeynep Kamil Maternity and Children’s Hospital, Istanbul, Turkey

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20151299

Keywords:

Follicular fluid, Calcium, Oocyte, ICSI, Reproductive outcomes

Abstract

Background: Quantification of follicular fluid (FF) composition can provide valuable information about the oocyte state which can be helpful in distinguishing high and low quality oocyte. In the absence of appropriate calcium signaling at fertilization, the embryo may fail to implant and/or develop to full term. We herein aimed to investigate whether FF levels of Ca2+ ion concentrations vary between patients who achieved pregnancy and those who did not following a standard ICSI treatment regimen, and hence to determine whether FF Ca2+ levels could predict reproductive outcomes in patients undergoing ICSI.

Methods: Patients seeking treatment for solely unexplained infertility were recruited in the period between June and December 2013 and were assigned to the groups according to their reproductive outcomes. Group 1 consisted of patients who conceived and Group 2 consisted of those who did not achieve pregnancy following ICSI. Parametric comparisons were performed using Student’s t-test. Categorical data were evaluated by using χ2 test.

Results: Groups 1 and 2 consisted of 34 and 66 patients, respectively. Although FF Ca2+ ion concentrations were higher in those who did not achieve any pregnancy, the difference did not reached to statistical significance level (7.6±0.4 mg/dl and 7.9±0.9 mg/dl respectively, p=0.070).

Conclusions: FF Ca2+ ion concentrations did not significantly differ between patients who achieved pregnancy and who did not followed ICSI attempts. Thus FF Ca2+ levels do not appear to be of benefit in predicting reproductive outcomes in unexplained infertility patients undergoing ICSI cycles.

References

Ferraretti AP, Goossens V, Kupka M, Bhattacharya S, de Mouzon J, Castilla JA, et al. Assisted reproductive technology in Europe, 2009: results generated from European registers by ESHRE. Hum Reprod. 2013;28:2318-31.

Haggarty P, Wood M, Ferguson E, Hoad G, Srikantharajah A, Milne E, et al. Fatty acid metabolism in human preimplantation embryos. Hum Reprod .2006;21:766–73.

Balaban B, Urman B. Effect of oocyte morphology on embryo development and implantation. Reprod Biomed Online 2006;12:608-15.

Patrizio P, Fragouli E, Bianchi V, Borini A, Wells D. Molecular methods for selection of the ideal oocyte. Reprod Biomed Online. 2007;15:346-53.

Dawson A, Griesinger G, Diedrich K. Screening oocytes by polar body biopsy. Reprod Biomed Online. 2006;13:104-9.

Revelli A, Piane LD, Casano S, Molinari E, Massobrio M, Rinaudo P. Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics. Reprod Biol Endocrinol .2009;7:40.

Nikiforaki D, Vanden Meerschaut F, Qian C, De Croo I, Lu Y, Deroo T, et al. Oocyte cryopreservation and in vitro culture affect calcium signalling during human fertilization. Hum Reprod 2014;29(1):29-40.

Kashir J, Heindryckx B, Jones C, De Sutter P, Parrington J, Coward K. Oocyte activation, phospholipase C zeta and human infertility. Hum Reprod Update. 2010;16:690–703.

Nomikos M, Swann K, Lai FA. Starting a new life: sperm PLC-zeta mobilizes the Ca2+ signal that induces egg activation and embryo development: an essential phospholipase C with implications for male infertility. Bioessays .2012;34:126–34.

Xu Z, Kopf GS, Schultz RM. Involvement of inositol 1,4,5-trisphosphate-mediated Ca2+ release in early and late events of mouse egg activation. Development .1994;120:1851–9.

Yu Y, Nomikos M, Theodoridou M, Nounesis G, Lai FA, Swann K. PLCz causes Ca(2+) oscillations in mouse eggs by targeting intracellular and not plasma membrane PI(4,5)P(2). Mol Biol Cell. 2012;23:371–80.

Jaffe JL. Sources of calcium in egg activation: a review and hypothesis. Dev Biol. 1983;99(2):265-76.

Hogben M, Parrington J, Shevchenko V, Swann K, Lai FA. Calcium oscillations, sperm factors and egg activation at fertilisation. J Mol Med (Berl). 1998;76(8):548-54.

Ducibella T, Schultz RM, Ozil JP. Role of calcium signals in early development. Semin Cell Dev Biol. 2006;17:324–32.

Santella L, Puppo A, Chun JT. The role of the actin cytoskeleton in calcium signaling in starfish oocytes. Int J Dev Biol .2008;52(5-6):571-84.

Santella L, Chun JT. Actin, more than just a housekeeping protein at the scene of fertilization. Sci China Life Sci. 2011;54:733-43.

Tola EN, Mungan MT, Uğuz AC, Naziroğlu M. Intracellular Ca2+ and antioxidant values induced positive effect on fertilisation ratio and oocyte quality of granulosa cells in patients undergoing in vitro fertilisation. Reprod Fertil Dev. 2013;25(5):746-52.

Tsafriri A. Ovulation as a tissue remodelling process. Proteolysis and cumulus expansion. Adv Exp Med Biol. 1995;377:121-40.

Tsafriri A, Reich R. Molecular aspects of mammalian ovulation. Exp Clin Endocrinol Diabetes. 1999;107:1-11.

Wallace M, Cottell E, Gibney MJ, McAuliffe FM, Wingfield M, Brennan L. An investigation into the relationship between the metabolic profile of follicular fluid, oocyte developmental potential, and implantation outcome. Fertil Steril. 2012;97:1078–84.

Özdamar Ö, Gün İ, Ertuğrul SS, Özden O, Sofuoğlu K. Does biochemical profile of follicular fluid in women undergoing assisted reproduction vary between the right and left ovaries?. Int J Reprod Contracept Obstet Gynecol .2015;4:1066-9.

Silberstein T, Saphier O, Paz-Tal O, Gonzalez L, Keefe DL, Trimarchi JR. Trace element concentrations in follicular fluid of small follicles differ from those in blood serum, and may represent long-term exposure. Fertil Steril. 2009;91(5):1771-4.

Azem F, Hanannel A, Wolf Y, Ben-Yosef D, Wagman I, Yovel I, et al. Divalent cation levels in serum and preovulatory follicular fluid of women undergoing in vitro fertilization embryo transfer. Gynecol Obstet Invest. 2004;57(2):86-9.

Makki M, Saboori E, Sabbaghi MA, Aram R, Fallahian MH, Peyghambari F, et al. Effects of selenium, calcium and calcium ionophore on human oocytes in vitro maturation in a chemically defined medium. Iran J Reprod Med. 2012;10(4):343-8.

Ebner T, Oppelt P, Wöber M, Staples P, Mayer RB, Sonnleitner U, et al. Treatment with Ca2+ ionophore improves embryo development and outcome in cases with previous developmental problems: a prospective multicenter study. Hum Reprod 2015;30(1):97-102.

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Published

2017-02-19

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Original Research Articles