DOI: http://dx.doi.org/10.18203/2320-1770.ijrcog20174044

Physico-analytical study of ghrita formulation indicated in Vataj Artava Dushti with special reference to hypomenorrhoea and oligomenorrhoea

Ajay Kumar, Neelam ., Sunita Suman, Riden Saxena

Abstract


Background: To establish safety profile for oral use of a classical Ayurvedic Ghrita formulation indicated in menstrual disorder known as Vataj Artava Dushti recent scientific techniques were used to fulfill the criteria of WHO guidelines for herbal drug formulations like heavy metal content and microbial load estimation, chromatography and others physic- analytical characteristic.

Methods: In the present study formulated ghrita sample was selected for estimation of refractive index, total ash value acid, iodine, saponification, loss on drying, PH, fat content, sugar content, heavy metals namely lead (Pb), cadmium (Cd), mercury (Hg), acid value, iodine value, by ICP-MAS, TLC and HPTLC.

Results: SEM- EDX shows presence of elements like Carbon, Oxygen and Aluminum, Lead, Calcium, Magnesium, Zinc. Refractive index 1.459, total ash 12%, saponification technique 55.26, Iodine value 4.32, acid value 6.6, PH 5.23 Loss on drying 0.32 fat 76.58% and sugar content 13.37%, Mercury <10 ppb, Lead 2.33 ppm, Cadmium 0.20 ppm. Total bacterial count 0.5×103, total fungal count <103. TLC and HPTLC results reveals presence of active compound.

Conclusions: The Physico-analytical, heavy metals, microbial and chromatographic study of the formulated ghrita follows the standard parameters. This study can be helpful for the future research regarding establishment of safety profile and therapeutic efficacy of ghrita formulations.


Keywords


Acid value, Ayurveda, Hypomenorrhoea, Iodine value, Oligomenorrhoea, Siddha ghrita, Saponification value, Vataj artava dushti

Full Text:

PDF

References


Acharya JT. SusrutaSamhita of Susruta with Nibandha Sangraha Commentary of Sri Dalhanacharya and Nyayachandrika Panjika of Sri Gayadasacharya. Reprint. Varanasi: Chaukambha Sanskrit Sansthan; 2009.p. 498.

Acharya YT. Caraka Samhita with Ayurveda Deepika Commentary of Chakrapanidatta. Reprint. Varanasi: Chaukambha publication; 2011. p.82.

Gupta KA. Astanga Sangrah hindi commentary Sarira sthana. Chapter 1/30, reprint 2005. Chaukhamba krishnadas academy, Varanasi. 2005;1:265.

Sharma PV. Dravyaguna vigyan vegetable drugs chaukhamba bharati academy reprint. 2009;2:75.253,298.

Kumar A, Suman S ayurvedic management of kashtartava WSR to primay dysmenorrhoea. WJPR. 2016;5(5);905-11.

Kumar A, Ayurvedic Parasurgical Procedures Commonly Used in Female Reproductive Diseases. IJHSR. 2016; 6(7):355-9.

Tiwari PV. Ayurvediya PrasutiTantra And StriRoga 2nd Edition., Chapter 2. reprint 2007;2:165-6.

Flegler SL, Heckman JW, Klomparens KL. Scanning and transmission Electron microscopy. WH. Freeman and company, Newyork; 1993.

Ayurvedic Formulary of India, Part II, New Delhi: Govt. of India Ministry of Health and Family Welfare, Dept. of ayush, 2003 p.75-6.

Indian Pharmacopoeia. Vol. 1. Ghaziabad, India: The Indian Pharmacopoeia Commission; 2014. Pharmaceutical methods; pp. 100–2.3.23.

AOAC- official methods of analysis of AOAC international, 18th edition 2005 edited by Dr. William Horwitz, chapter 9, P.35-6 and chapter 9.1.09 P19-22.

Rao RN, Talluri MVNK. An overview of recent applications of inductively coupled plasma-mass spectrometry (ICP-MS) in determination of inorganic impurities in drugs and pharmaceuticals. Journal of Pharmaceut Biomed Anal. 2007;43(1):1-13.

Gilman JC. A manual of soil fungi. 2nd ed. New Delhi: Oxford and IBH Publishing Corporation; 1975:220.

Wagner H, and Baldt S. plant drug analysis, A thin layer chromatography Atlas IInd edition 1996.

Sethi PD, High performance thin layer chromatography quantative analysis of Pharmaceutical formulations. Delhi, 1st edition 1996

Shankar D, Unnikrishnan PM, Venkatasubramanian P. Need to develop inter-cultural standards for quality, safety and efficacy of traditional Indian systems of medicine. Current Sci. 2007;92(11):1499-1505.

Bent S. Herbal medicine in the United States: review of efficacy, safety, and regulation: grand rounds at University of California, San Francisco Medical Center. Journal of General Internal Medicine. 2008;23(6):854–859.

Bruins MR, Kapil S, Oehme FW. Microbial resistance to metals in the environment. Ecotoxicol. Environ. Saf. 2000;45:198-207.

Nies DH. Microbial heavy metal resistance. Appl. Environ. Microbiol. 1999;51:730-50.

Saxena R, Azad CS, Shrinet K. Effect of heavy metal pollutants on human population: An Overview. IRJMST. 2016;7(3).

Saper RB, Kales SN, Paquin J, Burns MJ, Eisenberg DM, Davis RB, Phillips RS. Heavy metal content of ayurvedic herbal medicine products. JAMA. 2004;292(23):2868-73.

Ernst E. Heavy metals in traditional Indian remedies. Eur J Clin Pharmacol. 2002;57(12):891-6.

Coton M, Delbés-Paus C, Irlinger F. Diversity and assessment of potential risk factors of Gram-negative isolates associated with French cheeses. Food Microbiol. 2012;29(1):88-98.

Pitt JI. What are mycotoxins? Australian Mycotoxin Newsletter. 1996;7(4):1.