Study of serum calcium and vitamin D levels with hormonal profile along with biochemical profile in women with polycystic ovary syndrome

Ilangovan Subashree, Umakant Ramchandra Valvekar, Geetha Prasad


Background: The polycystic ovary syndrome (PCOS) is one of the commonest human endocrinopathies and is increasingly recognized as a variant of the metabolic syndrome in women with the characteristic features of insulin resistance, central obesity, impaired glucose metabolism, dyslipidemia, and hypertension.

Methods: This study is mainly focused on study of parameters like gonadotropin hormonal profile, serum vitamin D and calcium levels in polycystic ovary disease (PCOD). The study comprised 45 clinically proven polycystic ovary disease patients in the age range of 19-34 years. The biochemical estimations carried out in the study were – Fasting Blood sugar, LH, FSH, prolactin, 25- OH vitamin D and calcium along with anthropometric data. The values obtained were compared with age matched equal number of healthy control female subjects from the same population.

Results: The serum concentration of calcium and vitamin D levels are decreased significantly (P <0.001) when compared to controls. Insulin resistance is predominantly seen in PCOS subjects. The study outlines the importance of insulin resistance, dyslipidemia, decreased serum calcium and vitamin D levels in PCOS subjects may be a cause for the progression of polycystic ovary syndrome.

Conclusions: In the present study vitamin D deficiency is highly prevalent in PCOS women from this area compared to control women. We also relations of vitamin D status with insulin sensitivity, HDL-C, and C-reactive protein in PCOS patients, which support the increasing evidence that vitamin D deficiency is associated with multiple metabolic risk factors in PCOS women. A high prevalence of vitamin D deficiency and low calcium levels were observed in PCOS women from our population when compared to controls. Insulin resistance was predominantly seen in PCOS subjects when compared with controls, indicating the association of vitamin D levels with insulin resistance.


25-OH vitamin D, Gonadotropin hormones, Hypovitaminosis, Polycystic ovary syndrome, Serum calcium

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Kumar AN, Naidu JN, Satyanarayana U, Ramalingam K, Anitha M. Metabolic and endocrine characteristics of Indian women with polycystic ovary syndrome. Int J Fertil Steril. 2016;10(1):22-8.

Hahn S, Haselhorst U, Tan S, Quadbeck B, Schmidt M, Roesler S, et al. Low serum 25-hydroxyvitamin D concentrations are associated with insulin resistance and obesity in women with polycystic ovary syndrome. Exp Clin Endocrinol Diabetes. 2006;114:577-83.

Da Silva Feuser CS, Barbosa JS, da Silva EB, de Medeiros SF. Current insights into gonadotropic pituitary function in the polycystic ovary syndrome. Asia Pac J Reprod. 2014;3(1):64-70.

Dunaif A, Segal KR, Futterweit W, Dobrjansky A. Profound peripheral insulin resistance, independent of obesity, in the polycystic ovary syndrome. Diabetes. 1989;38(9):1165-74.

Holick MF. Vitamin D deficiency. N Engl J Med 2007;357:266-81.

Li HW, Brereton RE, Anderson RA. Vitamin D deficiency is common and associated with metabolic risk factors in patients with polycystic ovary syndrome. Metab Clin Exp. 2011;60:1475-81.

Holick MF. Vitamin D: importance in the prevention of cancers, type 1 diabetes, heart disease, and osteoporosis. Am J Clin Nutr. 2004;79:362-71.

Naidu JN, Swapna GN, Kumar AN, Krishnamma M, Anitha M. Importance of elevated insulin resistance, dyslipidemia and status of antioxidant vitamins in polycystic ovary disease. Free Radicals and Antioxidants. 2013;3(1):17-9.

Kumar AN, Naidu JN, Stayanarayanac U, Anitha M. Past, Present and Future of Insulin Gene and Its Related Genes In Relation To Polycystic Ovary Syndrome. J Mol Genet Med. 2014;8:107.

Yildizhan R, Kurdoglu M, Adali E. Serum 25-hydroxyvitamin D concentrations in obese and non-obese women with polycystic ovary syndrome. Arch Gynecol Obstet. 2009;280:559-63.

Lamberg BA. Glucose metabolism in thyroid disease. Acta Med Scand. 1965;178:351-62

Kumar AN, Satyanarayana U, Naidu JN, Ramalingam K, Anitha M. Comparison of Lipid Profile, Thyroid Profile, Glycaemic Status, Sex Hormonal Levels, 25-OH Vitamin D and Oxidative Stress Status in Obese and Non Obese Women with Polycystic Ovary Syndrome before and after Treatment with Metformin. J Pharm Biomed Sci. 2015;05(07):572-82.

The Rotterdam ESHRE/ASRM-Sponsored Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome (PCOS). Hum Reprod. 2004;19(1):41-7.

Wehr E, Trummer O, Giuliani A. Vitamin D associated polymorphisms are related to insulin resistance and vitamin D deficiency in PCOS. Eur J Endocrinol. 2011;164:741-9.

Kumar AN, Satyanarayana U, Naidu JN, Ramalingam K, Anitha M. Vitamin D receptor gene polymorphism in obese and non-obese Indian women with polycystic ovary syndrome. Int J Current Res. 2015;7(8):19686-91.

Shoelson SE, Herrero L, Naaz A. Obesity, inflammation, and insulin resistance. Gastroenterol. 2007;132:2169-80.

Mattson L, Culberg G, Hamberger L, Samsioe G, Silfverstolpe G. Lipid metabolism in women with polycystic ovary syndrome: possible implications for an increased risk of coronary heart disease. Fertil Steril. 1984;42:579-84.

Wild R, Painter P, Coulson P, Carruth K, Ranney G. Lipoprotein lipid concentrations and cardiovascular risk in women with polycystic ovary syndrome. J Clin Endocrinol Metab. 1985;61:946-51.

Talbott E, Clerici A, Berga SL, Kuller L, Guzick D, Detre K, et al. Adverse lipid and coronary heart disease risk profiles in young women with polycystic ovary syndrome: results of a case-control study. J Clin Epidemiol. 1998;51:415-22.

Saha S, Sarkar C, Biswas SC, Karim R. Correlation between serum lipid profile and carotid intima-media thickness in polycystic ovarian syndrome. Indian J Clinical Biochem. 2008;23(3):262-6.

Maestro B, Molero S, Bajo S. Transcriptional activation of the human insulin receptor gene by 1,25-dihydroxyvitamin D3. Cell Biochem Funct. 2002;20:227-32.

Mahmoudi T, Gourabi H, Ashrafi M. Calciotropic hormones, insulin resistance, and the polycystic ovary syndrome. Fertil Steril. 2010;93:1208-14.

Milner RD, Hales CN. The role of calcium and magnesium in insulin secretion from rabbit pancreas studied in vitro. Diabetologia. 1967;3:47-9.

Kayaniyil S, Vieth R, Harris SB. Association of 25(OH)D and PTH with metabolic syndrome and its traditional and nontraditional components. J Clin Endocrinol Metab. 2011;96:168-75.

Maestro B, Davila N, Carranza MC, Calle C. Identification of a vitamin D response element in the human insulin receptor gene promoter. J Steroid Biochem Mol Biol. 2003;84:223-30.

Wehr E, Pilz S, Schweighofer N, Giuliani A, Kopera D, Pieber T, et al. Association of hypovitaminosis D with metabolic disturbances in polycystic ovary syndrome. European J Endocrinol. 2009;161:575-82.

Srouji SS, Pagan YL, D Amato F, Dabela A, Jimenez Y, Supko JG, et al. Pharmacokinetic factors contribute to the inverse relationship between luteinizing hormone and body mass index in polycystic ovarian syndrome. J Clin Endocrinol Metab. 2007;92(4):1347-52.

Serafim C, da Silva Feuser. Current insights into gonadotropic pituitary function in the polycystic ovary syndrome Asian Pacific J Reproduct. 2014;3(1):64-70.

Kalsum A, Jalali S. Role of hyperprolactinemia in fertility. Pak J Med Res. 2002;41:1-5.

Nizam K, Memon N, Devrajani BR. Outcome of treatment with lisuride in hyperprolactinemic infertile women. J Liaquat Univ Med Hlth Sci. 2008;7(2):120-3.