Study on semen analysis in the evaluation of male infertility in coastal Karnataka, India


  • Pradeep Ganiga Department of Obstetrics and Gynecology, A. J. Institute of Medical Sciences and Research Center, Mangaluru, Karnataka, India
  • Shivakumar Pujeri Department of Obstetrics and Gynecology, A. J. Institute of Medical Sciences and Research Center, Mangaluru, Karnataka, India



Coitus frequency, Liquefaction, Semen analysis, Sperm concentration, Sperm morphology


Background: According to current recommendation a couple has to be evaluated if they fail to achieve pregnancy within one year of unprotected regular intercourse. The current incidence of infertility is around 15% which is increasing because of various factors including delay in attaining first pregnancy. The male partner evaluation includes detailed history and examination including the sexual history and semen analysis. Observational study performed on patients with primary and secondary infertility. The detailed semen analysis evaluation done in addition to notifying brief sexual history and chronic medical ailments like diabetes.

Method: After proper counselling, and obtaining consent, the patients were made to collect the semen sample in a room attached to the andrology lab. Detailed evaluation of each sample was done. Prior to this procedure, relevant information like occupation, age of the patient, duration of abstinence, coitus frequency and presence of chronic medical ailments like, diabetes mellitus and chronic hypertension were noted.

Results: The study, done in a tertiary care centre in coastal Karnataka, revealed that a majority of the patients were in the age group of 25-30 years with a larger group of patients having liquefaction time of <20minutes and 25% were in the oligospermia group.

Conclusions: Semen analysis is an indispensable diagnostic tool in the evaluation of the male partner of infertile couples in addition to brief sexual history and occupational history.


Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: a review of literature. J Human Reprod Sci. 2015;8(4):191.

Lalitha C, Sayee R, Apoorva D. Environmental/occupational factors and seasonality of birth-male infertility. World academy of science, engineering and technology. Int J Med Health Sci. 2015;2(8).

Esteves SC. Clinical relevance of routine semen analysis and controversies surrounding the 2010 World Health Organization criteria for semen examination. Int Braz J Urol. 2014;40(4):433-53.

Jajoo S, Kalyani KR. Prevalence of abnormal semen analysis in patients of infertility at a rural setup in Central India. Int J Reprod Contracept Obstet Gynecol. 20169;2(2):161-4.

Jain A, Ambrish PI, Hoogar MB, Dhar R, Wani A, Agrwal S. Role of semen analysis in the diagnosis of infertility at a tertiary care centre in Western India: a prospective study. Int J Reprod Contracept Obstet Gynecol. 2017;5(7):2389-91.

Abarikwu SO. Causes and risk factors for male-factor infertility in Nigeria: a review. Afr J Reprod Health. 2013;17(4):150-66.

Perlis N, Lo KC, Grober ED, Spencer L, Jarvi K. Coital frequency and infertility: which male factors predict less frequent coitus among infertile couples?. Fertil Steril. 2013;100(2):511-5.

Pellestor F, Girardet A, Andreo B. Effect of long abstinence periods on human sperm quality. Int J Fertil Menopausal Stud. 1994;39(5):278-82.

P R Pant. Factors affecting male infertility. J Instit Med. 2010;32(2).

Sharma R, Harlev A, Agarwal A, Esteves, SC. Cigarette smoking and semen quality: A new meta- analysis examining the effect of the 2010 WHO laboratory methods for the examination of human semen. Eur Urol. 2016;70(4):635-45.

Diallo MS, Diallo AS, Fotso P, Diallo Y, Diao B, Faye O. Semen Abnormality Patterns and Parameters in Male Partners of Infertile Couples in Dakar (Senegal). Open J Urol. 2015;5(09):155.

Ugwunja EP, Ugwa NC, Prevalence of low sperm count and abnormal semen parameters in male partners of women consulting at infertility clinic in Abakaliki, Nigeria. Afr J Reprod Health. 2005;12(1):67-73.

Damsgaard J, Joensen UN, Carlsen E, Erenpreiss J, Jensen MB, Matulevicius V, et al. Varicocele is associated with impaired semen quality and reproductive hormone levels: a study of 7035 healthy young men from six European countries. Eur Uro. 2016;70(6):1019-29.

Nallella KP, Sharma RK, Aziz N, Agarwal A. Significance of sperm characteristics in the evaluation of male infertility. Fertil Steril. 2006;85(3):629-34.

Harris ID, Fronczak C, Roth L, Fertility and the aging male. Rev Urol. 2011;13(4):e184-90.

Karabulut A, Tekin A, Alterations in the morphology and motility of spermatozoa: relation with total sperm count Pam Med J. 2013;6(1):1-4.

World Health Organization Department of reproductive health and research. WHO Laboratory Manual for the examination and processing of human semen 5th edition, Geneva, Switzerland: WHO 2010.






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