Estimation of maternal serum vitamin D levels and its correlation with gestational diabetes mellitus

Anupriya Narain, Alka Goel, Parul Goyal


Background: Several studies in the past two decades have proved beyond doubt that there is a high prevalence of hypovitaminosis D worldwide including India which has almost taken the shape of a pandemic. The study aims to determine the prevalence of hypovitaminosis D in the pregnant population of Delhi and its correlation with gestational diabetes mellitus.

Methods: This prospective cross sectional observational study was conducted at the Department of Obstetrics and Gynaecology, Postgraduate Institute of Medical Education and Research (PGIMER), Dr R.M.L Hospital, New Delhi over a period of 1 year and 4 months. Four hundred term patients were enrolled randomly from amongst the patients admitted in labor room or antenatal ward at PGIMER, Dr. Ram Manohar Lohia Hospital who fulfilled the inclusion and exclusion criteria. 3-4ml of fasting blood samples were collected of all the enrolled women and were analysed for total serum calcium levels and serum vitamin D levels. History of presence of gestational diabetes mellitus was noted along with any treatment history. The 25(OH)D levels for each subject was recorded and correlation of vitamin D levels with gestational diabetes mellitus was analysed. Statistical analysis was done using ANOVA/ Kruska Wallis test and Chi-Square test/ Fisher’s exact test. A p value of <0.05 was considered statistically significant.

Results: The overall prevalence of vitamin D deficiency in the present study was 86.25%. The overall mean serum vitamin D level was 14.06±9.43 ng/ml. Thirty five out of 37 GDM patients had hypovitaminosis D i.e. 94.59%. The prevalence of hypovitaminosis D among non-GDM population was 85.40%. The association between GDM and Vitamin D deficiency was statistically non- significant in the present study with p value=0.186.

Conclusions: There is a very high prevalence of hypovitaminosis D in pregnant females attending present institute i.e. 86.25%. Although 94.59 % of Gestational Diabetes Mellitus patients had vitamin D deficiency, there is no statistically significant association between Gestational diabetes mellitus and serum vitamin D levels.


Gestational diabetes mellitus, Hypovitaminosis D, Vitamin D deficiency

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Sachan A, Gupta R, Das V, Agarwal A, Awasthi P K, Bhatia V. High prevalence of vitamin D deficiency among pregnant women and their newborns in northern India. Am J Clin Nutr. 2005;81:1060-4.

Hollis BW, Wagner CL. Vitamin D requirements during lactation: high-dose maternal supplementation as therapy to prevent hypovitaminosis D for both the mother and the nursing infant. Am J Clin Nutr.2004;80:1752S-8S.

American Diabetes Association. Standards of medical care in diabetes. Dia Care.2014;37(1):S14-S80.

Holick MF. Vitamin D: importance in the prevention of cancers, type 1 diabetes, heart disease, and osteoporosis. Am J Clin Nutr. 2004;79:362-371.

Pittas AG, Lau J, Hu FB, Dawson-Hughes B. The role of vitamin D and calcium in type 2 diabetes. A systematic review and meta-analysis. J Clin Endocrinol Metab. 2007;92:2017-29.

Maestro B, Molero S, Bajo S, Davila N, Calle C. Transcriptional activation of the human insulin receptor gene by 1,25-dihydroxyvitamin D(3). Cell Biochem Funct. 2002;20:227-32.

Kazemi A, Sharifi F, Jafari N, Mousavinasab N. High prevalence of vitamin D deficiency among pregnant women and their newborns in an Iranian population. J Women's Health (Larchmt). 2009 June;18(6):835-9.

Farrant HJ, Krishnaveni GV, Hill JC. Vitamin D insufficiency is common in Indian mothers but is not associated with gestational diabetes or variation in newborn size. Eur J Clin Nutr. 2009;63(5):646-52.

Baker AM, Haeri S, Camargo CA, Jr, Stuebe AM, Boggess KA. First-trimester maternal vitamin D status and risk for gestational diabetes (GDM) a nested case–control study. Diabetes Metab Res Rev. 2012;28:164-8.

Makgoba M, Nelson SM, Savvidou M, Messow CM, Nicolaides K, Sattar N. First-trimester circulating 25-hydroxyvitamin D levels and development of gestational diabetes mellitus. Diabetes Care. 2011;34:1091-3.

Kumari A, Mitra S, Tiwari HC, Srivastav R, Hypovitaminosis D in pregnancy and its correlation with preeclampsia and gestational diabetes mellitus. Int J Reprod Contracept Obstet Gynaecol. 2017;6:890-6.

Mojibian M, Soheilykhah S, Fallah Zadah MA, Moghadam MJ. The effects of vitamin D supplementation on maternal and neonatal outcome: a randomised clinical trial. Iran J Reprod Med. 2015 Nov; 13(11):687-96.

Aghajafari F, Nagulesapillai T, Ronksley P, Tough S, O'Beirne M, Rabi D. Association between maternal serum 25-hydroxyvitamin D level and pregnancy and neonatal outcomes: systematic review and meta-analysis of observational studies. Bri Med J. 2013;346:f1169.

Parlea L, Bromberg IL, Feig DS, Vieth R, Merman E, Lipscombe LL. Association between serum 25- hydroxyvitamin D in early pregnancy and risk of gestational diabetes mellitus. Diabet Med. 2012;29:e25-32.

Zhang C, Qiu C, Hu FB, David RM, Van Dam RM, Bralley A, et al. Maternal plasma 25-hydroxyvitamin D concentrations and the risk for gestational diabetes mellitus. PloS one. 2008 Nov 18;3(11):e3753.