Correlation between amniotic fluid lamellar body concentrations at different gestational ages and respiratory distress syndrome

Amruta C. C.; Hema A. Patil

doi:10.18203/2320-1770.ijrcog20185421

Authors

Amruta C. C. Department of Obstetrics and Gynecology, SDM College of Medical Sciences, Dharwad, India
Hema A. Patil Department of Obstetrics and Gynecology, SDM College of Medical Sciences, Dharwad, India

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20185421

Keywords:

Lamellar body counts, Preterm, RDS

Abstract

Background: Respiratory distress syndrome (RDS) also known as hyaline membrane disease is the most common cause of respiratory failure in neonates. The risk of RDS rises in prematurity due to decreased production of surfactant. Lamellar bodies (LB) are storage form of surfactant and are actively secreted into the alveolar space and hence into the amniotic fluid. The objective of the present study was to find out correlation between amniotic fluid lamellar body concentrations (LBC) at different gestational ages and respiratory distress syndrome

Methods: The study was a prospective observational study conducted over a period of 1 year at department of OBG, SDM college of medical sciences, Dharwad. All women undergoing caesarean section were included in the study. 5 ml of amniotic fluid was collected at the time of Caesarean section and sent to laboratory, Lamellar body concentrations (LBC) was counted in auto analyzer by platelet impedance counting. Incidence of RDS at different gestational age with an LBC cut off 40,500 was calculated.

Results: Among 300 patients studied, Respiratory distress was seen in 116 (41.3%) of patients. LBC cut off of 40,500 was chosen. Out of 140 preterm babies, 121 (86.8%) had lamellar body count less than 40,500 and 19 (13.5%) had more than 40,500. There is significant correlation between decreasing lamellar body count in preterms and increasing incidence of RDS.

Conclusions: LBC count increases with increasing gestational ages and is inversely proportional to the incidence of RDS in preterm newborns.

Metrics

Metrics Loading ...

References

Angus D, Linde-Zwirble W, Clermont G, Griffin MF, Clark RH. Epidemiology of neonatal respiratory failure in the United States: Projections from California and NewYork. Am J Respir Crit Care Med. 2001;164(7):1154-60.

Fakhoury G, Daikoku NH, Benser J, Dubin NH. Lamellar body concentrations and the prediction of fetal pulmonary maturity. Am J Obstet Gynecol. 1994;170(1):72-6

Lafler DJ, Mendoza A. Laboratory testing to assess fetal lung maturity. Lab Med. 2001;32(7):393-6.

Smith BT. Fibroblast –Pnuemocyte factor: Am J Physiol Lung Cell Mol Physiol 1989;257(4):174-8

Grenache DG, GronowskiAM. Fetal lung maturity. Clin Biochem.2006;39(1):1-10.

Khazardoost S, Yahyazadeh H, Borna S, Sohrabvand F, Yahyazadeh N, Amini E. Amniotic fluid lamellar body count and its sensitivity and specificity in evaluating of fetal lung maturity: J Obstet Gynaecol. 2005;25(3):257-9

Ross GE, Bever FN, Uddin Z, Hockman EM, Herman BA. Decreased laboratory testing for lecithin-to-sphingomyelin ratio and phosphatidylglycerol after fetal lung maturity assessment from lamellar body count in amniotic fluid. JAOA. 2002;102(8):8-14

Roiz‐Hernández J, Navarro‐Solis E, Carreón‐Valdéz E. Lamellar bodies as a diagnostic test of fetal lung maturity. Int J Gynecol Obstet. 2002;77(3):217-21.

Haymond S, Luzzi VI, Parvin CA,Gronowski AM. A direct comparison between lamellar body counts and fluorescent polarization methods for predicting respiratory distress syndrome.Am J ClinPathol.2006;126(6):894-9.

Chapman JF, Ashwood ER, Feld R, Wu AH. Evaluation of two-dimensional cytometric lamellar body counts on the ADVIA® 120 hematology system for estimation of fetal lung maturation. Clinica Chimica Acta. 2004;340(1-2):85-92.

Bahasadri S, Changizi N. Association between lamellar body count and respiratory distress in neonates. Saudi Med J. 2005;26(9):1414-6.