Beta-fibrinogen (-455 G/A) and Integrin beta-3 (PLA1/A2) polymorphisms and recurrent pregnancy loss in Gaza strip-Palestine

Manal G. Al-Astal, Fadel A. Sharif


Background: This study was conducted in order to determine the relationship between the common polymorphisms in β-fibrinogen -455 G/A (rs1800790) and Integrin beta-3 T196C (PLA1/A2, rs5918) and recurrent pregnancy loss (RPL) in a group of Palestinian women residing in Gaza strip.

Methods: The study presented here is a retrospective association study with a case-control design. The study population consisted of 102 women who suffered from RPL and 97 healthy women matched for age and without a previous history of RPL. DNA extracted from blood samples of all subjects was investigated for the β-fibrinogen (-455 G>A) and Integrin beta-3 (PLA1/A2) polymorphisms using PCR-RFLP and PCR-SSP, respectively.

Results: The study revealed that β–fibrinogen -455G/A minor allele frequency (allele A) and its homozygous genotype (AA) were not significantly different between the RPL and the control groups. Likewise, there was no significant difference in the distribution of the ITGB-3 variant allele (PLA2) or PLA2/A2 genotype between the two groups. β–fibrinogen AA genotype was evident in 3.9% of the patients and in 4.1% of the controls (P-value = 0.94). The frequency of PLA2/A2 was observed in 2.0% of the patients and in 2.1% of the controls (P-value = 0.96).

Conclusion: Results of the present study indicate that there is no significant association between the β–fibrinogen
(455 G>A) or Integrin beta-3 (PLA1/A2) common polymorphisms and the occurrence of RPL in Gaza strip. This non-significant relation indicates that the investigated polymorphisms do not constitute a tangible risk for RPL in our population. Therefore, it is not recommended to consider these two polymorphisms when screening for inherited thrombophilia in Gaza strip.


β–fibrinogen, Integrin beta-3, gene polymorphism, RPL, Gaza Strip, Palestine

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