Manfort, a blend from plant extracts used for infertility treatment and improvement of testicular histology
Keywords:Flavonoids, Manfort, Polyphenols, Safety, Testosterone, Testis
Background: Flavonoids and polyphenols are groups of natural substances have variable phenolic configurations, many benefits as anti-inflammatory and anti-oxidants, and have many protective roles against male reproductive system disorders. Objective of this study was to study the safety as well as the efficacy of a blend from some plants extracts with precise ratios and rich with flavonoids and polyphenols named in this study as “Manfort” on the safety, fertility, and testicular histology in the male mice.
Methods: Firstly, some mice were used to evaluate the safety of Manfort and the levels of the testosterone in the serum of the treated animals with Manfort using biochemical analysis. Also, the efficacy of the Manfort on the histological architectures of the treated testis was evaluated using histological techniques.
Results: The mice treated with Manfort did not show any signs of mortality, toxicities and blood contents changes. Furthermore, testosterone levels in the serum elevated after administration with Manfort twice a day for 21 days compared with the non-treated mice. Additionally, the histological structures of the testis improved in the mice treated with Manfort compared with that in the non-treated animals.
Conclusions: In general flavonoids and polyphenols, which were found in the Manfort diet in a large amount, have important role in as antioxidant, anti-inflammatory and improve the fertility in the male mice. In the future these data might be very important to manufacture a drug composed from safe natural products for infertility and intractable diseases treatment.
Jenkinson C, Petroczi A, Naughton DP. Effects of ary Components on Testosterone Metabolism via UDP-Glucuronosyltransferase. Front Endocrinol (Lausanne). 2013;4:80.
Dai R, Jacobson KA, Robinson RC, Friedman FK. Differential effects of flavonoids on testosterone-metabolizing cytochrome P450s. Life Sci. 1997;61(7):PL75-80.
Budin SB, Abdul Rahman WZ, Jubaidi FF Yusof NLM, Taib IS, Zainalabidin S. Roselle (Hibiscus sabdiriffa) polyphenol-rich extract prevents testicular damage of diabetic mice. J Appl Pharma Sci. 2018;8(2):065-70.
Abarikwu SO, Olufemi PD, Lawrence CJ, Wekere FC, Ochulor AC, Barikuma AM. Rutin, an antioxidant flavonoid, induces glutathione and glutathione peroxidase activities to protect against ethanol effects in cadmium-induced oxidative stress in the testis of adult rats. Androl. 2017;49(7):e12696.
Samie A, Sedaghat R, Baluchnejadmojarad T, Roghani M. Hesperetin, a citrus flavonoid, attenuates testicular damage in diabetic rats via inhibition of oxidative stress, inflammation, and apoptosis. Life Sci. 2018;1(210):132-9.
Zhao H, Song L, Huang W, Liu J, Yuan D, Wang Y, et al. Total flavonoids of Epimedium reduce ageing-related oxidative DNA damage in testis of rats via p53-dependent pathway. Androl. 2017;49(10):1-3.
Ahmed NZ, Ibrahim SR, Ahmed-Farid OA. Quercetin and Apigenin of Cymbopogon citratus mediate inhibition of HCT-116 and PC-3 cell cycle progression and ameliorate Doxorubicin-induced testicular dysfunction in male rats. Biomed Res Ther. 2018;5(7):2466-79.
Ribeiro GG, Pessôa LR, de Abreu MD, Corrêa LB, Pereira AA, Chagas MA, et al. Taro flour (Colocasia esculenta) increases testosterone levels and gametogenic epithelium of Wistar rats. Journal of developmental origins of health and disease. 2018;9(4):373-6.
Jana K, Yin X, Schiffer RB, Chen JJ, Pandey AK, Stocco DM, et al. Chrysin, a natural flavonoid enhances steroidogenesis and steroidogenic acute regulatory protein gene expression in mouse Leydig cells. J Endocrinol. 2008;197(2):315-23.
He QX, Zhang YG, Hu X, Guo YT. Tea polyphenols protects the testis following testicular torsion/detorsion in rats. Zhonghua Nan Ke Xue. 2010;16(7):615-8.
Patel AS, Leong JY, Ramos L, Ramasamy R. Testosterone is a contraceptive and should not be used in men who desire fertility. World J Mens Health. 2019;37(1):45-54.
Walker WH. Non-classical actions of testosterone and spermatogenesis. Philos Trans R Soc Lond B Biol Sci. 2010;365:1557-69.
Smith LB, Walker WH. The regulation of spermatogenesis by androgens. Semin Cell Dev Biol. 2014;30:2-13.
Walker WH, Cheng J. FSH and testosterone signaling in Sertoli cells. Repro. 2005;130:15-28.
Beecher GR. Overview of dietary flavonoids: nomenclature, occurrence and intake. J Nutr. 2003;133:3248S-3254S.
Rojas DP, Pandey AK. Natural Compounds to Counteract Testosterone Depletion in Aging. J Steroids Horm Sci. 2014;5:e112.
Patel AS, Leong JY, Ramos L, Ramasamy R. Testosterone is a contraceptive and should not be used in men who desire fertility. World J Men's Health. 2019;37(1):45-54.
Galati G, O'Brien PJ. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic Biol Med. 2004;37(3):287-303.
Yuan D, Wang H, He H, Jia L, He Y, Wang T, et al. Protective effects of total flavonoids from Epimedium on the male mouse reproductive system against cyclophosphamide-induced oxidative injury by up-regulating the expressions of SOD3 and GPX1. Phytother Res. 2014;28(1):88-97.