Levels of prolactin, progesterone, estradiol, luteinizing hormone and follicle stimulating hormone in infertile women in Calabar, Nigeria

I. K. Isong, Z. A. Okhormhe, I. Eze-Bassey, D. C. Okpokam, C. A. O. Usoro


Background: Infertility is defined as the inability of a couple to achieve conception despite frequent unprotected, well timed sexual intercourse for one year duration. Therefore the aim of our study was to estimate the levels of progesterone, estradiol, luteinizing hormone, follicle stimulating hormone in infertile women.

Methods: One hundred and two (102) volunteers infertile women; aged 20-45 years were recruited from the University of Calabar Teaching Hospital. They were further divided into three groups based on their prolactin levels; namely those with normal ovarian hormones value (normal); (n=32), those with prolactin values between 25 – 60ng/ml (moderate) (n=46) and those with prolactin values >60ng/ml (high); (n=24). Sixty (60) apparently healthy, age matched women were also selected to serve as the control group. Sera samples were obtained from the subjects and the following parameters were measured using DRG Enzyme Linked Immunosorbent Assay; prolactin, progesterone, estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH).

Results: In this study, the prolactin and E2 values were significantly (p<0.05) higher in the high group compared to the normal ovarian profile and moderate group, while progesterone levels were significantly higher in the normal group compared to the moderate and high groups. There was no significant difference in the values of LH and FSH among the groups (p>0.05). There was a positive correlation between E2 and prolactin, progesterone and FSH (r=0.239; 0.368 and 0.217 respectively, p˂0.05) and a strong positive correlation between FSH and LH (r=0.677) in the infertile women.

Conclusions: There was a positive correlation between E2 and progesterone with reduced concentration of FSH in women with normal, moderate and high prolactin concentrations. Therefore, there is need for anti-oxidant therapy or supplements for these subjects.


Progesterone, Estradiol, Luteinizing hormone, Follicle stimulating hormone, Infertile women

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