A study of microbiological analysis and its sensitivity pattern of postoperative wound infections in obstetrics and gynecology department: retrospective study in tertiary institute in Mangalore, Karnataka, India


  • Pramodha Mallik Shagun Department of Obstetrics and Gynecology, AJ Institute of Medical Science and Research Centre Mangalore, Karnataka, India
  • Chaitra S. Department of Obstetrics and Gynecology, AJ Institute of Medical Science and Research Centre Mangalore, Karnataka, India




Polymicrobial, Postoperative, Surgical site infection


Background: Infections acquired in hospitals are classified as nosocomial infections. Most of these infections are related to surgery, usually at the post-operative wound site. It is also termed Surgical Site Infection (SSI). Staphylococcus aureus is the documented most common organism that infect surgical wounds. This study was focused on identifying the most common microorganism documented in post-operative wound sepsis in abdomen hysterectomy surgery of obstetrics and gynaecology in a tertiary hospital in Mangalore. The aim of this study was to identify postoperative infection and to determine their current antimicrobial resistance to commonly prescribed drugs.

Methods: Materials and Methods: This retrospective research was carried out in tertiary hospital with a sample size of 90 wound samples, whose records span from Jan 2014-Jan 2018.  The study involved obtaining records of subjects with cases of contamination and isolation of organisms that frequently infect post-operative wound site in abdomen hysterectomy surgery.

Results: A total of 79 bacterial isolates were obtained from 95 wound culture samples. In 41 cases, the isolated culture were mono microbial, 39 cultures were polymicrobial whereas no growth was obtained in 15 cases. The results showed that Staphylococcus Aureus was the most frequently isolated organism (48.1%), followed by Pseudomonas aeruginosa (18.98%) and E. coli (17.72%).

Conclusions: The study is therefore important in identifying those at risk of post-operative wound sepsis and the probable causative organisms. This will be useful in the choice of prophylactic antibiotics for treatment of high-risk patients, thereby setting up an empirical antibiogram for use.


Naik G, Deshpande S. A study on surgical site infections caused by staphylococcus aureus with a special search for methicillin-resistant isolates. J Clin Diagnost Res. 2011;5:502-8.

Avalos-Bock S. Knocking out nosocomial infections. Nurs. 2019;34(11):24-5.

Anusha S, Vijaya LD, Pallavi K, Manavalan R. An epidemiological study of surgical wound infections in a surgical unit of tertiary care Teaching Hospital. Indian J Phar Pract. 2010;4:8-13.

Biadglegne F, Abera B, Alem A. Bacterial isolates from wound infection and their antimicrobial susceptibility pattern in Felege Hiwot Referral Hospital, North West Ethiopia. Ethiop J Health Sci. 2009;19:173-7.

Insan NG, Payal N, Singh M, Yadav A, Chaudhary BL, Srivastava A. Post-operative wound infection: bacteriology and antibiotic sensitivity pattern. IJCRR. 2013;5(13):74-9.

Raza M, Ranabhat A. Antimicrobial susceptibility patterns of the bacterial isolates in post-operative wound infections in a tertiary care hospital, Kathmandu, Nepal. Open J Med Microbiol. 2013;3:159-63.

Schwartz SI, Comshires G, Spencer FC, Dally GN, Fischer J, Galloway AC. Principles of surgery. 9th edition. Chapter 6L: surgical infections. NY: McGraw-Hill companies; 2010.

Gautam R, Acharya A, Nepal HP, Shrestha S. Antibiotic susceptibility pattern of bacterial isolates from wound infection in Chitwan Medical College Teaching Hospital, Chitwan, Nepal. IJBAR. 2013;4(4):248-52.

Bessa LJ, Fazii P, Giulio MD. Bacterial isolates from infected wounds and their antibiotic susceptibility pattern: some remarks about wound infection. Int. Wound J. 2015;12(1):47-52.

Manikandan C, Amasth A. Antibiotic susceptibility of bacterial strains isolated from wound infectionpatients in Pattukkottai, Tamil Nadu, India. Int J Curr Microbiol App Sci. 2013;2(6):195-203.

Anil K. Study and use of antimicrobials in post- operative wound infections in gastrointestinal surgeries. Indo Am J Pharm Res. 2013;3:4.

Amoran OE, Sogebi AO, Fatugase OM. Rates and risk factors associated with surgical site infections in a tertiary care center in South-Western Nigeria. Int J Trop Dis Health. 2013;3(1):25-36.

Majeed MT, Izhar M. Glycopeptides sensitivity pattern in staphylococci isolated from clinical specimens in a tertiary care hospital. Ann KE Med Coll. 2015;11:263-71.






Original Research Articles