DOI: http://dx.doi.org/10.18203/2320-1770.ijrcog20202304

Effect of one-time dextran-polyacrylamide polymer matrixes treatment on female reproductive function

Valentyna A. Sribna, Oksana N. Kaleinikova, Yulia I. Kuziv, Alena A. Vinogradova Anyk, Igor N. Karvatskiy, Tetiana Y. Voznesenskaya, Taras V. Blashkiv, Natalia V. Kutsevol

Abstract


Background: recently, it has been proved that copolymers with dextran cores and grafted polyacrylamide are effective in photodynamic and chemotherapy. However, further research is needed to define correct dosage and to assess the risks. Thus, animal studies are becoming more relevant to determine the effect of the treatment of such drug nano-systems on female reproductive function in particular.

Methods: a technique for estimation of pre- and post-implantation death rates, in vitro meotic maturation of oocytes, double fluorescent vital assay and statistical analysis were used. The effects of a one-time treatment of different doses of dextran-polyacrylamide matrices and silver (Ag)-nanoparticles-dextran-polyacrylamide (AgNPs-D-PAA) on reproductive function, namely on 1) the number of oocytes isolated from one ovary and the meiotic maturation of such oocytes in vitro; 2) the indicators of cell viability of the cells of follicular environment of oocytes (FEO) and the cells of inguinal lymph nodes (ILN); 3) the pre- and post-implantation mortality rates and the number of live newborns (pups) were investigated in female mice.

Results: no significant changes in the number of oocytes isolated from one ovary and meiotic maturation of such ovarian oocytes in vitro, the number of living cells of follicular environment of oocytes  and the number of such cells with morphological signs of apoptosis and necrosis, pre- and post-implantation mortality rates of embryos and the number of live newborns (pups) have been established under conditions of one-time treatment with dextran-polyacrylamide at doses of 0.39 mg/kg and 3.90 mg/kg and Ag-nanoparticles-dextran-polyacrylamide at doses of 0.20 mg/kg and 2.00 mg/kg.

Conclusions: branched polymer systems (dextran-polyacrylamide (D-PAA) polymer matrices) are promising materials for use in next-generation medicine.


Keywords


Apoptosis, Dextran-polyacrylamide polymers, Meiotic maturation of oocytes, Necrosis, Pre- and post-implantation mortality

Full Text:

PDF

References


Telegeev G, Kutsevol N, Chumachenko V, Naumenko A, Telegeeva P, Filipchenko S, et al. Dextran-Polyacrylamide as Matrices for Creation of Anticancer Nanocomposite. Int J Polym Sci. 2017;1(9):1-9.

Kutsevol N, Naumenko A, Harahuts Yu, Chumachenko V, Shton I, Shishko E, et al. New hybrid composites for photodynamic therapy: synthesis, characterization and biological study. Appl Nanosci. 2019;9(5):881-8.

Kutsevol N, Guenet J, Melnik N, Sarazin D, Rochas C. Solution properties of dextran-polyacrylamide graft copolymers. Polymer. 2006;47(6):2061-8.

Kutsevol N, Bezugla T, Bezuglyi M, Rawiso M. Branched dextran-graft-polyacrylamide copolymers as perspective materials for nanotechnology. Macromolecular Symposia. 2012;318(1):82-90.

Shimizu S, Eguchi Y, Kamiike W, Akao Y, Kosaka H, Hasegawa J, et al. Involvement of ICE family proteases in apoptosis induced by reoxygenation of hypoxic hepatocytes. Am J Physiol. 1996;271(6-1):949-58.

Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited. FASEBJ. 2008;22(3):659-61.

Lytvynenko A, Rieznichenko L, Sribna V, Stupchuk M, Grushka N, Shepel A, et. al. Functional status of reproductive system under treatment of silver nanoparticles in female mice. Int J Reprod Contracept Obstet Gynecol. 2017;6(5):1713-20.

Sribna V, Kaleynykova O, Grushka N, Blashkiv T, Voznesenska T, Yanchiy R. Resumption of meiotic maturation of oocytes, pre- and postimplantation mortality of embryos under ten-time intravenous treatment of silver nanoparticles in mice. Int J Reprod Contracept Obstet Gynecol. 2018;7(11):4360-5.

Zhang X, Liu Z, Shen W, Gurunathan S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci. 2016;17(9):1534.

Khan U, Saleh T, Wahab A, Khan M, Khan D, Khan W, et al. Nano-silver: new ageless and versatile biomedical therapeutic scaffold. Int J Nanomed. 2018;2(13):733-62.

Tiedemann D, Taylor U, Rehbock C, Jakobi J, Klein S, Kues W, et al. Reprotoxicity of gold, silver, and gold-silver alloy nanoparticles on mammalian gametes. Analyst. 2014;139(5):931-42.

Hsin Y, Chen C, Huang S, Shih T, Lai P, Chueh P. The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicol Lett. 2008;179(3):130-9.

Piao M, Kang K, Lee I, Kim H, Kim S, Choi J, et al. Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett. 2011;201(1):92-100.

Kim S, Ryu D. Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues. J Appl Toxicol. 2013;33(2):78-89.

Laban G, Nies L, Turco R, Bickham J, Sepúlveda M. The effects of silver nanoparticles on fathead minnow (Pimephalespromelas) embryos. Ecotoxicol. 2010;19(1):185-95.

Chernousova S, Epple M. Silver as antibacterial agent: ion, nanoparticle, and metal. Angew Chem Int Ed Engl. 2013;52(6):1636-53.