A retrospective study on analyzing the role of oocyte maturation arrest as a causative factor for infertility in unexplained infertility


  • Mahalakshmi S. Founder Director, HOD, ARC International Fertility Center, Chennai, Tamil Nadu, India
  • J. Lokeshwari Fellowship Scholar, ARC International Fertility Center, Chennai, Tamil Nadu, India
  • Nidhi Sharma Department of Obstetrics and Gynecology, Saveetha Institute of Medical Sciences, Chennai, Tamil Nadu, India




Estrodial level, Immature oocytes, Invitro fertilization, Unexplained fertility


Background: Birth is often referred to as “the miracle of life” and very rightly so since successful pregnancy represents a complex, a highly coordinated succession of biological processes. In this regard, it is remarkable that pregnancy ever occurs at all. Infertility is a reproductive system disorder affecting 10.7% to 15.5% of couples. Highly heterogeneous in etiology. Investigating the cause is necessary for guiding treatment options. Aim of the study was to determine the percentage of immature oocytes retrieved at the metaphase 1 stage and germinal vesicle stage and its outcome.

Methods: Total 30 cases were included for this retrospective analysis of data done at ARC fertility center, Saveetha Medical College. This study analyzed the data of patients with unexplained infertility who underwent ICSI centers (affiliated to Saveetha University) meeting inclusion criteria and willing to participate in the study.

Results: The mean number of fertilized oocytes among those with more than 25% immature oocyte proportion was 1.18 while among those with less than 25% immature oocyte proportion was 4.13. The chance of fertilization of the oocyte increases when the percentage of immature oocyte was less than or equal to 25%. The association was found to be statistically significant with p-value <0.05.

Conclusions: Thus, some intrinsic oocyte factor could be involved in causing maturation arrest in such couples and resulting in multiple failed IVF cycles.so this oocyte factor could be a major cause in many cases of unexplained infertility.


Andrews WW, Goldenberg RL, Hauth JC, Cliver SP, Conner M, Goepfert AR. Endometrial microbial colonization and plasma cell endometritis after spontaneous or indicated preterm versus term delivery. Am J Obstet Gynecol. 2005;193:739-45.

Arici A, Seli E, Senturk LM, Gutierrez LS, Oral E, Taylor HS. Interleukin-8 in the human endometrium. J Clin Endocrinol Metab. 2001;83:1783-7.

Asimakopoulos B, Abu-Hassan D, Metzen E, Al-Hasani S, Diedrich K, Nikolettos N. The levels of steroid hormones and cytokines in individual follicles are not associated with the fertilization outcome after intracytoplasmic sperm injection. Fertil Steril. 2008;90:60-4.

Axeman P, Ching C, Machungo F, Osman NB, Bergstrom S. Intrauterine infections and their association with stillbirth and preterm birth in Maputo, Mozambique. Gynecol Obstet Invest. 2003;35:108-13.

Balasch J, Martinez-Roman S, Creus M, Campo E, Fortuny A, Vanrell J. Schistosomiasis: an unusual case of tubal infertility. Hum Reprod. 1995;10:1725-7.

Barnhart KT, Stolpen A, Pretorius ES, Malamud D. Distribution of a spermicide containing nonoxynol-9 in the vaginal canal and the upper female reproductive tract. Hum Reprod. 2001;16:1151-4.

Barron AL, Rank RG, Moses EB. Immune response in mice infected in the genital tract with mouse pneumonitis agent (Chlamydia trachomatis biovar). Infect Immun. 2006;44:82-5.

Barroso G, Barrionuevo M, Rao P, Graham L, Danforth D, Huey S, et al. Vascular endothelial growth factor, nitric oxide, and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients. Fertil Steril. 2009;72:1024-6.

Chun SY, Eisenhauer KM, Kubo M, Hsueh AJ. Interleukin-1 β suppresses apoptosis in rat ovarian follicles by increasing nitric oxide production. Endocrinol. 2005;136:3120-7.

Cicinelli E, De Ziegler D, Nicoletti R, Tinelli R, Salinas N, Resta L, et al. Poor reliability of vaginal and endocervical cultures for evaluating microbiology of endometrial cavity in women with chronic endometritis. Gynecol Obstet Invest. 2010;68:108-15.

Cintron RD, Wortham JW, Acosta A. The association of semen factors with the recovery of Ureaplasma Urealyticum. Fertil Steril. 1991;36:648-52.

Cohen C, Mugo N, Astete S, Redondo R, Manhart L, Kiehlbauch J, et al. Detection of mycoplasma genitalium in women with laparoscopically diagnosed acute salpingitis. Sex Transm Infect. 2006;81:463-6.

Dean D, Powers V. Persistent chlamydia trachomatis infections resist apoptotic stimuli. Infect Immun. 2001;69:2442-7.

Debattista J, Gazzard CM, Wood RN, Allan JA, Allan JM, Scarman A, et al. Interaction of microbiology and pathology in women undergoing investigations for infertility. Infect Dis Obstet Gynecol. 2001;12:135-41.

Essbase PE, Udo EE, Al-Sharhan M, Grudzinskas JG. Prophylactic antibiotics and endocervical microbial inoculation of the endometrium at embryo transfer. Lancet. 2008;354:651-2.

Eggert-Kruse W, Gerhard I, Naher H, Tilgen W, Runnebaum B. Chlamydial infection-a female and/or male infertility factor? Fertil Steril. 2010;53:1037-43.






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