DOI: http://dx.doi.org/10.18203/2320-1770.ijrcog20210731

Assessment of the pregnancy rates using sequential day 3 and day 5 embryo transfer in IVF/ ICSI patients

Priti Agrawal, Rishi Agrawal, Anandi Lobo

Abstract


Background: To assess the pregnancy rates using sequential day 3 and day 5 embryo transfer in IVF/ ICSI patients.

Methods: This prospective study was conducted in Aarogya Hospital and test tube baby Centre, Raipur from 1st January 2013 to 30th November 2019. Total 100 patients undergoing IVF/ICSI in the study period were offered sequential transfer.

Results: Our fertilization rates were 80% with 85.7% grade I embryos on day 3. Blastocyst formation rate was 71.42%. Cycle cancellation rates were nil. Clinical pregnancy rates per retrieval cycle were 50% and implantation rates were 24% with acceptable multiple pregnancy rates of 12%.

Conclusions: We advocate that this technique is useful in all patients having good quality embryos in adequate number for double transfer as this optimizes the chance of selection of the most viable embryo for transfer which is probably the key for a successful IVF program.

 


Keywords


Blastocyst transfer, Embryo transfer, Repeated implantation failure, Sequential embryo transfer

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References


Simon A, Laufer N: Assessment and treatment of repeated implantation failure (RIF); J Assist Reprod Genet. 2012;29:1227-39.

Gardner DK, School Craft WB, Wagley L, Schlenker T, Stevens J, Hesla J. A prospective randomized trial of blastocyst culture and transfer in in-vitro fertilization, Human Reprod. 1998;13:3434-40.

Ismail Madkour WA, Noah B, Zaheer H, Bahr A A, Abdelhamid A M.S, Shaeer M et al: Does sequential embryo transfer improve pregnancy rate in patients with repeated implantation failure? A randomized control studies. J Middle East Fertility Society 2015.

Goto S, Takcbayashi K, Shiotani M, Fuji Wara M, Hirose M, Noda Y. Effectiveness of 2 step consecutive transfer J Repord Med. 2003;48:370-4.

Dalal R, Mishra A, Pai HD, Palshetkar N. A prospective trial comparing sequential day 3/ day 5 transfer with cleavage stage transfer and blastocyst stage transfer. J Minimal Stimulation IVF. 2015; 2:30-6.

Fang C, Huang R, Li TT, Jia L, Li LL, Liang XY. Day -2 and day -3 sequential transfer improves pregnancy rate in patients with repeated IVF - embryo transfer failure: a retrospective case - control study. Reproductive Bio Medicine online. 2013;26: 30-5.

Loutraids D, Drakakis P, Dallianidis K, Bletsa SR, Millingos S, Doumplis N, et al. A double embryo transfer on day 2 and 4 or 5 improves pregnancy outcome In patients with good embryos but repeated failures in IVF or ICSI. J Clin Exp Obstet Gynecol. 2004;31(1):63-6.

Tehraninejad E S, Raisi E, Ghaleh F B, Rashidi B H, Aziminekoo E, Kalantari V, Haghollahi F, Shariat M, The sequential embryo transfer compared to blastocyst embryo transfer in in vitro fertilization (IVF) cycle in patients with the three repeated consecutive IVF. A randomized controlled trial, Gynecol Endocrinol. 2019;35(11):955-9.

Kader AA, Choi A, Orief Y, Agrawal A. Factors affecting the outcome of human blastocyst vitrification. J Reproductive Biology and Endocrinol. 2009;7:99:1-11.