Successful intracytoplasmic sperm injection with testicular spermatozoa using pentoxifylline and HOS from a male with necrozoospermia: a case report

Authors

  • Usharani Nampalli Department of Embryology, Garbhagudi IVF Centre, Bangalore, Karnataka, India
  • Syed Fyzullah Department of Embryology, Garbhagudi IVF Centre, Bangalore, Karnataka, India
  • Chinmayie R. Department of Reproductive Medicine, Garbhagudi IVF Centre Bangalore, Karnataka, India
  • Asha Srinivasan Vijay Department of Reproductive Medicine, Garbhagudi IVF Centre Bangalore, Karnataka, India
  • Rajsri T. R. Department of Community Medicine, Evidencian Research Association, Karnataka, India

DOI:

https://doi.org/10.18203/2320-1770.ijrcog20211544

Keywords:

Necrozoospermia, Intracytoplasmic sperm injection, Testicular sperm, HOS, Pentoxyfylline

Abstract

The study was done to compare fertilization, cleavage, and pregnancy outcome of a man affected with necro zoospermia  using pentoxifylline and a hypoosmotic swelling (HOS) test to select viable spermatozoa from testicular biopsy specimens. A patient was diagnosed with necrozoospermia by semen analysis, and testicular spermatozoa were injected intracytoplasmically, using pentoxifylline and HOS (Hypo osmotic swelling test) and the following ICSI results were observed. Significantly higher fertilization rates (pentoxifylline 100% vs HOS 80%) were observed. There was no significant difference observed in cleavage rates among both groups. Finally, a healthy female infant with birth weight 3 kg was delivered at the second frozen embryo transfer cycle.it was found that selecting viable spermatozoa using pentoxifylline was more effective in fertilization and pregnancies than obtaining it through a HOS test. ICSI is effective for necrozoospermic man.

References

World Health Organization‎. WHO laboratory manual for the examination and processing of human semen. 5th ed. 2010.

Negri L, Patrizio P, Albani E, Morenghi E, Benaglia R, Desgro M, et al. ICSI outcome is significantly better with testicular spermatozoa in patients with necrozoospermia: a retrospective study. Gynecol Endocrinol. 2014;30(1):48-52.

Casper RF, Meriano JS, Jarvi KA, Cowan L, Lucato ML. The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril. 1996;65(5):972-6.

Mangoli V, Mangoli R, Dandekar S, Suri K, Desai S. Selection of viable spermatozoa from testicular biopsies: a comparative study between pentoxifylline and hypoosmotic swelling test. Fertil Steril. 2011;95(2):631-4.

Tesarik J, Mendoza C, Carreras A. Effects of phosphodiesterase inhibitors caffeine and pentoxifylline on spontaneous and stimulus-induced acrosome reactions in human sperm. Fertil Steril. 1992;58(6):1185-90.

Downloads

Published

2021-04-23

Issue

Section

Case Reports